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Transcriptome Analysis Reveals the Molecular Mechanism of Pseudomonas with Different Adhesion Abilities on Tilapia

Liumin Zhuang1, Chen Song1, Yunru Wei1

  • 1Institute of Food Science and Technology, College of Biological Science and Engineering, Fuzhou University, Fuzhou 350108, China.

Foods (Basel, Switzerland)
|March 13, 2025
PubMed

Insights

Pseudomonas adhesion to tilapia intestines drives spoilage. Environmental salt (NaCl) stress alters Pseudomonas adhesion, impacting spoilage bacteria and volatile compound production, thus influencing fish quality.

Area of Science:

  • Microbiology
  • Food Science
  • Molecular Biology

Background:

  • Pseudomonas species are significant contributors to fish spoilage.
  • The adhesion capability of Pseudomonas to fish intestinal mucus is a key factor in spoilage.
  • Environmental factors can modulate bacterial adhesion and subsequent spoilage potential.

Purpose of the Study:

  • To investigate the molecular mechanisms by which Pseudomonas adhesion influences tilapia spoilage.
  • To determine how environmental factors, specifically sodium chloride (NaCl), affect Pseudomonas adhesion and gene expression.
  • To identify key genes and regulatory networks involved in Pseudomonas adhesion to tilapia intestinal mucus.

Main Methods:

  • Exposure of Pseudomonas strains (PS01 and LP-3) to 3.5% NaCl stress.
  • Analysis of changes in bacterial adhesion ability and expression of adhesion-related genes (e.g., flgC, fliC, cheB).
  • Transcriptomics analysis to identify regulatory networks (flagellar assembly, chemotaxis, quorum sensing, etc.) and key adhesion genes.

Main Results:

  • NaCl stress enhanced adhesion in low-adhesion strain PS01 but decreased it in high-adhesion strain LP-3.
  • Altered gene expression correlated with changes in adhesion ability.
  • High-adhesion LP-3 increased spoilage bacteria (Nocardioides, Cloacibacterium) and volatile compounds, leading to greater spoilage; reduced adhesion diminished this effect.

Conclusions:

  • Pseudomonas adhesion to tilapia intestinal mucus is a critical determinant of spoilage potential.
  • NaCl stress significantly impacts Pseudomonas adhesion mechanisms and gene expression.
  • An adhesion regulatory network involving multiple bacterial systems was identified, highlighting 10 key adhesion-related genes.
Keywords:
NaCl regulationPseudomonasadhesion genesspoilage

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