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Near-infrared (NIR) Fluorophores in Cancer Bioimaging and Therapy

Libo Yan1, Weiming Zhang2, Daogang Wang3

  • 1School of Stomatology and Ophthalmology, Xianning Medical College, Hubei University of Science and Technology, Xianning, 437000, China.

Current Medicinal Chemistry
|March 13, 2025
PubMed

Keywords:
Malignancybioimagingindocyanine greennear-infraredphotodynamic therapyphotothermal therapy

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Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.