Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Assembly of the Lipid Bilayer in the ER01:28

Assembly of the Lipid Bilayer in the ER

3.1K
Biological membranes are more than just a barrier separating cell cytoplasm from the outside environment. They are highly dynamic and help maintain the integrity and physiological stability of the cells as well as membrane-bound organelles. Membranes also play vital roles in cell-to-cell and intracellular communication.
A large chunk of any biological membrane is composed of phospholipids. These lipids have a heterogeneous distribution across different subcellular organelles and even between...
3.1K
Golgi Apparatus01:49

Golgi Apparatus

89.8K
As they leave the Endoplasmic Reticulum (ER), properly folded and assembled proteins are selectively packaged into vesicles. These vesicles are transported by microtubule-based motor proteins and fuse together to form vesicular tubular clusters, subsequently arriving at the Golgi apparatus, a eukaryotic endomembrane organelle that often has a distinctive ribbon-like appearance.
89.8K
Membrane Domains01:18

Membrane Domains

5.3K
The membrane domains concentrate specific lipids and proteins at one place within the membrane, which helps in cell signaling, adhesion, and other critical cellular processes. These domains can differ in size, composition, function, and lifespan.
Protein Domains
The membrane comprises a group of distinct proteins responsible for carrying out a cell's specific function. For example, the plasma membrane of the human sperm, or a single germ cell, contains a unique set of proteins in the...
5.3K
The Early Endosome: Endocytosis of Transferrin01:28

The Early Endosome: Endocytosis of Transferrin

3.2K
Essential proteins such as insulin or low-density lipoprotein (LDL) and micronutrients such as iron enter a eukaryotic cell through receptor-mediated endocytosis. Subsequently, the early endosomes fuse with the vesicles containing such receptor-ligand complexes and play a vital role in sorting the incoming ligands and receptors. While the ligands are either degraded inside the vesicle or released into the cytosol, their receptors are returned to the plasma membrane for further rounds of...
3.2K
Asymmetric Lipid Bilayer01:35

Asymmetric Lipid Bilayer

7.1K
Biological membranes show uneven distribution of different types of lipids in the inner and outer layers, resulting in transverse asymmetric membranes. The treatment of the erythrocyte membrane with the enzyme phospholipase confirmed the asymmetric nature of the lipid bilayer. The enzyme hydrolyzes lipids into fatty acids and hydrophilic groups. The phospholipase acts only on the outer layer of the membrane, while the inner layer remains intact. The phospholipase treatment resulted in 80%...
7.1K
Introduction to Membrane Traffic01:44

Introduction to Membrane Traffic

6.7K
The ER, Golgi apparatus, endosomes, and lysosomes work in tandem to modify, sort, and package proteins and lipids. An integrated membrane trafficking network facilitates the back and forth shuttling of molecules within different organelles in the same cell or across the cell membrane.
The transport of soluble and membrane proteins is mediated by transport vesicles that collect cargo from one cellular compartment and deliver it to another by fusing with the target organelle membrane. The Rab...
6.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Trans-kingdom coupling of redox signaling to environmental cell stress responses through multiphase partitioning.

bioRxiv : the preprint server for biology·2026
Same author

<i>Blautia wexlerae</i> Transforms Dietary Fatty Acids to Activate Enteroendocrine Signaling and Improve Metabolic Health in Mice and Humans.

bioRxiv : the preprint server for biology·2026
Same author

Palmitoylated caveolin-1 enables endosome sorting of complex sphingolipids.

bioRxiv : the preprint server for biology·2025
Same author

Intestinal injury and repair: insights from hyaline fibromatosis syndrome.

EMBO molecular medicine·2025
Same author

The Genetic Architecture of Congenital Diarrhea and Enteropathy.

The New England journal of medicine·2025
Same author

SLO co-opts host cell glycosphingolipids to access cholesterol-rich lipid rafts for enhanced pore formation and cytotoxicity.

mBio·2025

Related Experiment Video

Updated: May 22, 2025

Quantitative and Qualitative Method for Sphingomyelin by LC-MS Using Two Stable Isotopically Labeled Sphingomyelin Species
08:53

Quantitative and Qualitative Method for Sphingomyelin by LC-MS Using Two Stable Isotopically Labeled Sphingomyelin Species

Published on: May 7, 2018

9.7K

Sorting of complex sphingolipids within the cellular endomembrane systems.

Victor O Svistunov1, Kigumbi J Ehrmann1, Wayne I Lencer1,2,3

  • 1Division of Gastroenterology, Hepatology and Nutrition, Boston Children's Hospital, Boston, MA, United States.

Frontiers in Cell and Developmental Biology
|March 13, 2025
PubMed
Summary
This summary is machine-generated.

Cellular membranes contain diverse lipids that must be sorted and transported. This review covers recent advances in understanding lipid sorting during endomembrane trafficking.

Keywords:
complex sphingolipidsendocytosismembrane curvaturemembrane nanodomainsmembrane raftsmembrane trafficking

More Related Videos

Expression, Purification, and Liposome Binding of Budding Yeast SNX-BAR Heterodimers
10:28

Expression, Purification, and Liposome Binding of Budding Yeast SNX-BAR Heterodimers

Published on: December 6, 2019

7.7K
Preparation of Human Tissues Embedded in Optimal Cutting Temperature Compound for Mass Spectrometry Analysis
09:09

Preparation of Human Tissues Embedded in Optimal Cutting Temperature Compound for Mass Spectrometry Analysis

Published on: April 27, 2021

2.0K

Related Experiment Videos

Last Updated: May 22, 2025

Quantitative and Qualitative Method for Sphingomyelin by LC-MS Using Two Stable Isotopically Labeled Sphingomyelin Species
08:53

Quantitative and Qualitative Method for Sphingomyelin by LC-MS Using Two Stable Isotopically Labeled Sphingomyelin Species

Published on: May 7, 2018

9.7K
Expression, Purification, and Liposome Binding of Budding Yeast SNX-BAR Heterodimers
10:28

Expression, Purification, and Liposome Binding of Budding Yeast SNX-BAR Heterodimers

Published on: December 6, 2019

7.7K
Preparation of Human Tissues Embedded in Optimal Cutting Temperature Compound for Mass Spectrometry Analysis
09:09

Preparation of Human Tissues Embedded in Optimal Cutting Temperature Compound for Mass Spectrometry Analysis

Published on: April 27, 2021

2.0K

Area of Science:

  • Cell Biology
  • Membrane Biology
  • Lipidomics

Background:

  • Cellular membranes are composed of a wide variety of structurally diverse lipid species.
  • Lipids are asymmetrically distributed across various membrane compartments within cells.
  • Specific lipid species necessitate vesicular transport to reach their designated subcellular locations.

Purpose of the Study:

  • To review recent advancements in the understanding of lipid sorting mechanisms.
  • To elucidate the processes involved in the transport of lipids within the endomembrane system.

Main Methods:

  • Literature review of recent studies on lipid sorting and trafficking.
  • Analysis of current research on vesicular transport of lipids.
  • Synthesis of findings related to endomembrane dynamics and lipid distribution.

Main Results:

  • Recent research has shed light on the complex mechanisms governing lipid sorting.
  • Understanding of how specific lipids are directed to distinct membrane compartments has advanced.
  • The role of vesicular trafficking in lipid distribution is increasingly recognized.

Conclusions:

  • Lipid sorting is a critical process for maintaining cellular membrane organization and function.
  • Continued research into lipid trafficking pathways is essential for a comprehensive understanding of cell biology.
  • Advances in this field have significant implications for understanding cellular homeostasis and disease.