Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Raman Spectroscopy: Overview01:20

Raman Spectroscopy: Overview

292
The underlying principle of Raman spectroscopy is based on the interaction between light and matter, specifically molecules' inelastic scattering of photons. When a monochromatic beam of light, typically from a laser source, interacts with a sample, most scattered light has the same frequency as the incident light. This is known as Rayleigh scattering.
However, a small fraction of the scattered light exhibits a frequency shift due to the exchange of energy between the incident photons and...
292

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Highly Sensitive Molecular Detection Using Tapered Fiber with Ag Nanoparticle Self-Assembled Film for Confined-Enhanced Raman Spectroscopy.

Analytical chemistry·2025
Same author

Assembly-Dependent Regulation of the Chiroptical Responses of Chiral Gold Nanoparticles.

ACS nano·2025
Same author

Accurate Droplet Manipulation on the Central Radiant Grating Array: Effective Interfacial Enrichment and Spatial Localization for Ultrasensitive Label-Free SERS Biosensing.

ACS sensors·2025
Same author

Time-Gated Raman Spectroscopy Combined with Deep Learning for Rapid, Label-Free Histopathological Discrimination of Gastric Cancer.

Analytical chemistry·2025
Same author

Label-Free SERS Analysis of Biological and Physical Information Heterogeneity of Nanoscale Extracellular Vesicle by Matching Specific Sizes of Enhanced Particles.

Small (Weinheim an der Bergstrasse, Germany)·2024
Same author

PDDA-Assisted Synthesis of Magnetic Fluorescent Fe<sub>3</sub>O<sub>4</sub>@SiO<sub>2</sub>-CQD Composites.

Langmuir : the ACS journal of surfaces and colloids·2024

Related Experiment Video

Updated: May 22, 2025

Observation and Analysis of Blinking Surface-enhanced Raman Scattering
05:52

Observation and Analysis of Blinking Surface-enhanced Raman Scattering

Published on: January 11, 2018

7.3K

Updated Insights Into the Mechanism of Salt-Induced Aggregation-Based Single-Molecule Surface-Enhanced Raman

Lingwei Li1, Ruiyuan Zhang1, Yu Guo1

  • 1Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology, Xi'an Jiaotong University, Xi'an, Shaanxi, 710049, P. R. China.

Advanced Science (Weinheim, Baden-Wurttemberg, Germany)
|March 13, 2025
PubMed
Summary

This study reveals the mechanism behind single molecule surface-enhanced Raman spectroscopy (SM-SERS) using a salt-gradient model. It shows how silver nanoparticle aggregation and AgCl formation enhance ultrahigh sensitivity for SM-SERS applications.

Keywords:
Ag colloidaggregation agentsingle moleculesurface‐enhanced Raman spectroscopy

More Related Videos

Author Spotlight: Single-Molecule Surface-Enhanced Raman Scattering Measurements Enabled by Plasmonic DNA Origami Nanoantennas
10:43

Author Spotlight: Single-Molecule Surface-Enhanced Raman Scattering Measurements Enabled by Plasmonic DNA Origami Nanoantennas

Published on: July 21, 2023

3.2K
Surface Enhanced Raman Spectroscopy Detection of Biomolecules Using EBL Fabricated Nanostructured Substrates
11:44

Surface Enhanced Raman Spectroscopy Detection of Biomolecules Using EBL Fabricated Nanostructured Substrates

Published on: March 20, 2015

20.2K

Related Experiment Videos

Last Updated: May 22, 2025

Observation and Analysis of Blinking Surface-enhanced Raman Scattering
05:52

Observation and Analysis of Blinking Surface-enhanced Raman Scattering

Published on: January 11, 2018

7.3K
Author Spotlight: Single-Molecule Surface-Enhanced Raman Scattering Measurements Enabled by Plasmonic DNA Origami Nanoantennas
10:43

Author Spotlight: Single-Molecule Surface-Enhanced Raman Scattering Measurements Enabled by Plasmonic DNA Origami Nanoantennas

Published on: July 21, 2023

3.2K
Surface Enhanced Raman Spectroscopy Detection of Biomolecules Using EBL Fabricated Nanostructured Substrates
11:44

Surface Enhanced Raman Spectroscopy Detection of Biomolecules Using EBL Fabricated Nanostructured Substrates

Published on: March 20, 2015

20.2K

Area of Science:

  • Analytical Chemistry
  • Materials Science
  • Spectroscopy

Background:

  • Single molecule surface-enhanced Raman spectroscopy (SM-SERS) shows great potential but lacks mechanistic understanding.
  • Commercial applications are limited due to incomplete knowledge of SM-SERS mechanisms.

Purpose of the Study:

  • To investigate the physical nature of SM-SERS using a salt-gradient model.
  • To elucidate the evolution of silver nanoparticles (Ag NPs) during salt-induced aggregation.
  • To update insights into the morphological, structural, and component changes of Ag NPs.

Main Methods:

  • Development and application of a salt-gradient model.
  • Observation of Ag NP evolution from dispersed to aggregated states.
  • Analysis of Ag NP dissolution, Ag+ ion release, and AgCl precipitation.

Main Results:

  • A gradient interface was observed, achieving single-molecule level sensitivity in Ag colloidal systems.
  • Unusual dissolution of Ag and release of Ag+ ions from Ag NPs were evidenced.
  • An active AgCl shell on Ag NPs was found to significantly improve SERS properties beyond aggregation effects.

Conclusions:

  • The study reveals the physical processes and nature of SM-SERS.
  • The formation of an AgCl shell is crucial for enhancing SERS properties.
  • This work provides a new strategy for practical SM-SERS applications by controlling NP surface states and activation compositions.