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Effect of the inhibitory material from male genital tract on natural killing activities
Abstract:
A male inhibitory material (HuSePl-Frl) was separated from normal human seminal plasma by gel filtration on Sephadex G-100. This material has high avidity for cell membranes of human red blood cells (RBC). In vitro, PHA-M-induced blast transformation of human lymphocytes is inhibited by small quantities of HuSePl-Frl. This inhibition was also evident when RBC coated with HuSePl-Frl is added to mitogen stimulated lymphocytes in cultures. Small quantities of HuSePl-Frl are inhibitory to natural killer (NK) cell activation. The modulating role of the HuSePl-Frl in preventing the spermatozoal rejection during fertilization is discussed.
Insights
A novel male inhibitory material (HuSePl-Frl) from human seminal plasma inhibits lymphocyte and natural killer cell activation. This finding suggests a role for HuSePl-Frl in preventing immune rejection during fertilization.
Area of Science:
- Immunology
- Reproductive Biology
- Biochemistry
Background:
- Human seminal plasma contains factors that modulate immune responses.
- Understanding these factors is crucial for reproductive health and immunology.
Purpose of the Study:
- To isolate and characterize a male inhibitory material from human seminal plasma.
- To investigate the effects of this material on lymphocyte and natural killer cell activity.
Main Methods:
- Gel filtration chromatography (Sephadex G-100) was used for isolation.
- In vitro assays were performed to assess inhibition of lymphocyte blast transformation and NK cell activation.
Main Results:
- A male inhibitory material (HuSePl-Frl) was successfully isolated.
- HuSePl-Frl demonstrated high avidity for red blood cell membranes.
- Small quantities of HuSePl-Frl inhibited PHA-M-induced lymphocyte blast transformation and NK cell activation.
Conclusions:
- HuSePl-Frl possesses significant immunomodulatory properties.
- The material may play a role in immune tolerance during fertilization by preventing spermatozoal rejection.