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Related Concept Videos

Labeling DNA Probes03:31

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
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In-situ Hybridization

In situ hybridization (ISH) is a technique used to detect and localize specific DNA or RNA molecules in cells, tissue, or tissue sections using a labeled probe. The technique was first used in 1969 for the investigation of nucleic acids. It is currently an essential tool in scientific research and clinical settings, especially for diagnostic purposes.
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A probe is a complementary strand of DNA or RNA that binds to corresponding nucleotide sequences in a cell. Many...

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Related Experiment Video

Updated: May 11, 2026

In Vitro Aggregation Assays Using Hyperphosphorylated Tau Protein
09:22

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Published on: January 2, 2015

In Situ Labeling of Pathogenic Tau Using Photo-Affinity Chemical Probes.

Pengju Nie1, You Wu1,2, John Robinson3

  • 1Chemical Biology Program, Memorial Sloan-Kettering Cancer Center, New York, New York 10065, United States.

ACS Chemical Biology
|March 13, 2025
PubMed
Summary
This summary is machine-generated.

Researchers developed novel photoaffinity probes to specifically label tau proteins in Alzheimer's disease (AD) models and patient brains. These tools aid in understanding tauopathies and developing targeted therapies for neurodegenerative diseases.

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Last Updated: May 11, 2026

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Published on: November 9, 2018

Area of Science:

  • Neuroscience
  • Biochemistry
  • Molecular Biology

Background:

  • Tau aggregation is a key factor in Alzheimer's disease (AD) pathogenesis.
  • Targeted isolation of pathogenic tau is crucial for understanding tauopathies and developing therapies.

Purpose of the Study:

  • To develop novel photoaffinity small molecular probes for in situ tau labeling.
  • To investigate the efficacy of these probes in cells and Alzheimer's disease (AD) patient brains.

Main Methods:

  • Design and synthesis of tau-specific photoaffinity probes (Tau-2 and Tau-4) based on PET tracer structures.
  • Validation in cell cultures, mouse models, and post-mortem AD brain tissues.
  • Proteomic analysis for verification of pathogenic tau isolation.

Main Results:

  • Tau-2 effectively labeled soluble tau in cellular and mouse models.
  • Tau-4 selectively bound high-molecular-weight tau aggregates in late-stage AD patient brains.
  • Proteomic analysis confirmed the specific isolation of pathogenic tau from AD brain samples.

Conclusions:

  • Photoaffinity probes offer a powerful method for investigating tau proteins in neurodegenerative diseases.
  • These probes facilitate the study of tau pathology and the development of targeted therapeutic strategies.
  • The developed probes show potential for advancing research into tauopathies and Alzheimer's disease.