Related Experiment Video
Updated: Jun 28, 2026
Hand Controlled Manipulation of Single Molecules via a Scanning Probe Microscope with a 3D Virtual Reality Interface
Published on: October 2, 2016
RmZHD Surface-Displayed Escherichia coli for High Efficiency and Low-Cost Degradation of Zearalenone
Haoxiang Chen1, Xiaoyu Wang1,2, Hui Ge3
1Key Laboratory of Biomedical Engineering of Fujian Province University/Research Center of Biomedical Engineering of Xiamen, Department of Biomaterials, College of Materials, State Key Laboratory of Vaccines for Infectious Diseases, Xiang An Biomedicine Laboratory, Xiamen University, Xiamen 361005, P. R. China.
Abstract:
Zearalenone (ZEN) is one of the most prevalent mycotoxins in the world, with estrogenic toxicity leading to significant annual economic losses and environmental pollution. RmZHD, a novel ZEN hydrolase, surpasses the efficiency of its predecessors but faces challenges in large-scale industrial application. In this work, an engineered Escherichia coli that can degrade zearalenone is constructed based on synthetic biology and surface display methods. It can degrade 94% of zearalenone at 30 °C in 1 h (the final concentration was 1.898 μg/mL) and effectively degrade ZEN-derived toxins, including zearalanone, β-zearalanol, α-zearalenol, and β-zearalenol. This engineered E. coli requires no additional manipulation for the surface display of RmZHD and is cost-effective to produce. Moreover, it exhibits the capability to degrade ZEN in maize feed while concurrently mitigating inflammation in animal reproductive and digestive organs. In summary, the engineered E. coli with surface-displayed RmZHD presents a novel approach for environmentally sustainable and industrial-scale treatment of ZEN.
