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Related Experiment Videos

Cell markers in stored blood.

E Fosse, J L Svennevig, M Harboe

    Injury
    |May 1, 1985
    PubMed
    Summary
    This summary is machine-generated.

    Stored blood transfusions impact lymphocyte cell markers. E-rosette formation significantly decreased, while immunoglobulin binding increased, affecting immune cell function in patients receiving transfusions.

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    Area of Science:

    • Immunology
    • Transfusion Medicine
    • Cell Biology

    Background:

    • Major injuries and surgeries often necessitate large volumes of stored blood transfusions.
    • Stored blood products undergo changes that can affect cellular components, particularly lymphocytes.

    Purpose of the Study:

    • To investigate the changes in common lymphocyte cell markers in whole blood stored for one week.
    • To assess the impact of microfiltration on these stored blood components.

    Main Methods:

    • Studied lymphocyte cell markers (E-rosette, non-specific esterase, surface immunoglobulin) in whole blood before and after one week of storage.
    • Evaluated cell marker changes before and after passage through a microfilter.
    • Monitored white blood cell and granulocyte counts.

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    Main Results:

    • A substantial reduction in total white blood cells was primarily due to granulocyte decrease.
    • E-rosette forming lymphocytes decreased significantly (69% to 17%) after 24 hours of storage.
    • Non-specific esterase activity in lymphocytes remained unchanged after one week.
    • Surface-bound immunoglobulin on lymphocytes increased due to secondary binding to Fc receptors.
    • EA-rosette formation remained unaltered; microfiltration caused minimal cell loss.

    Conclusions:

    • Stored whole blood exhibits significant alterations in lymphocyte surface markers, notably a decrease in E-rosette formation and an increase in immunoglobulin binding.
    • These changes may have implications for immune function in transfusion recipients.
    • Microfiltration appears to minimally impact cell recovery and viability.