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Injectable Human Acellular Adipose Matrix with Crosslinked Hyaluronic Acid and Carboxymethyl Cellulose Gels for Soft
Si Youn Kim1, Jung Ki Lee2, Soon Won Jung2
1Department of Plastic and Reconstructive Surgery, Institute for Human Tissue Restoration, Yonsei University College of Medicine, 50-1 Yonsei-ro, Seodaemun-gu, Seoul, 03722, Republic of Korea.
Tissue Engineering and Regenerative Medicine
|March 13, 2025
Summary
A novel acellular adipose matrix (AAM) filler promotes new adipose tissue formation and superior volume retention compared to traditional fillers. This biomaterial shows promise for soft tissue augmentation and addressing volume deficits.
Area of Science:
- Biomaterials Science
- Regenerative Medicine
- Tissue Engineering
Background:
- Fillers are used for volume restoration but struggle to promote adipogenesis.
- A human acellular adipose matrix (AAM) combined with crosslinked hyaluronic acid (HA) and carboxymethyl cellulose (CMC) gels was investigated for volume maintenance and adipogenesis.
Purpose of the Study:
- To evaluate a novel AAM filler for its ability to maintain volume and promote adipose tissue formation.
- To compare the performance of the AAM filler with conventional HA fillers and native adipose tissue.
Main Methods:
- AAM was prepared and characterized for residual fat and cells.
- In vitro studies assessed human adipose-derived stem cell (hADSC) proliferation and adipogenic differentiation on AAM.
- An injectable AAM filler was formulated and tested in a nude mice model over 12 weeks, evaluating volume retention, cell incorporation, and adipogenesis.
Main Results:
- AAM effectively removed fat and cells, promoting hADSC viability and differentiation in vitro.
- The AAM filler demonstrated significantly higher viscosity than HA filler.
- In vivo, the AAM filler outperformed HA filler and adipose tissue in volume retention, cell incorporation, and new adipose tissue formation.
Conclusions:
- The AAM filler is a promising biomaterial for soft tissue augmentation.
- It offers advantages in both volume retention and the promotion of adipogenesis.
