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Evidence of SUFBC2D directly deliver Fe-S cluster to apo- NITRITE REDUCTASE1 (NIR1)
Zhaoyang Liu1, Yuting Cheng1, Long Zhang1
1Joint International Research Laboratory of Agriculture and Agri-Product Safety of the Ministry of Education, Yangzhou University, Yangzhou, 225009, China; College of Bioscience and Biotechnology, Yangzhou University, Yangzhou, 225009, China.
Abstract:
Fe-S clusters are ancient, ubiquitous, and highly essential prosthetic groups of many proteins. According to current understanding, Fe-S clusters are biosynthesized on scaffold, and transferred to carrier proteins, thereafter, they will be inserted into apo-proteins. However, it was also suggested that the scaffold complex SUFBC2D can directly deliver Fe-S cluster to apo-proteins. In this study, we combined biotin ligase-based proximity labeling with Yeast two-hybrid (Y2H) assay, Bimolecular fluorescence complementation (BiFC) assay and pull-down assay, identified that chloroplast Fe-S protein NIR1 directly interact with SUFC, suggesting that NIR1 may directly receive the Fe-S clusters from SUFBC2D. Thus, we provided evidence to support the exist of a new Fe-S clusters delivery pathway in chloroplast.
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