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Published on: April 11, 2016
Extraction optimization, purification, characterization, and hypolipidemic activities of polysaccharide from pumpkin
Haizhao Song1, Yanhui Zhang1, Qianqian Huang1
1College of Food Science and Engineering, Nanjing University of Finance and Economics/Collaborative Innovation Center for Modern Grain Circulation and Safety, Nanjing 210023, China.
Pumpkin polysaccharides, optimized via ultrasound-assisted extraction, show significant potential as natural hypolipidemic agents. The purified fraction PPS3 exhibits potent in vitro lipid-lowering effects, highlighting pumpkin as a valuable source for functional ingredients.
Area of Science:
- Food Science and Technology
- Biochemistry
- Natural Product Chemistry
Background:
- Pumpkin polysaccharides are gaining attention for their biological properties.
- Extraction and characterization of these polysaccharides are crucial for understanding their applications.
Purpose of the Study:
- To optimize the extraction of pumpkin polysaccharides using ultrasound-assisted methods.
- To characterize the purified pumpkin polysaccharide fraction (PPS3).
- To evaluate the in vitro hypolipidemic activity of PPS3.
Main Methods:
- Ultrasound-assisted extraction optimized by response surface methodology.
- Purification using DEAE Sepharose Fast Flow and Sephadex G-100 chromatography.
- Structural analysis including molecular mass determination and monosaccharide composition.
- In vitro assessment of hypolipidemic activities.
Main Results:
- Optimal extraction conditions: 1:60 (g/mL) solid-liquid ratio, 1.9 h, 62°C, 286 W.
- Achieved total carbohydrate content of 71.14 ± 1.53%.
- Purified fraction PPS3 (MW 5.96 × 10⁴ Da) characterized by specific monosaccharide composition and a →4)-α-D-GalpA-6-OMe-(1→ backbone.
- PPS3 demonstrated significant in vitro hypolipidemic activity.
Conclusions:
- Ultrasound-assisted extraction provides an efficient method for obtaining pumpkin polysaccharides.
- The characterized pumpkin polysaccharide PPS3 possesses a unique structure.
- PPS3 shows promise as a natural agent for managing lipid levels.
