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Monitoring Kinase and Phosphatase Activities Through the Cell Cycle by Ratiometric FRET

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Monitoring Molecular Interactions with Cell Membranes Using Time-Dependent Second Harmonic Generation Microscopy.

Prakash Hamal¹, Sushant P Sahu^2,3, Peter P Piers¹

¹Department of Chemistry, Louisiana State University, Baton Rouge, Louisiana 70803, United States.

|March 14, 2025

Summary

This summary is machine-generated.

This study used time-resolved second harmonic generation (SHG) microscopy to track how drug molecules interact with lung cancer cell membranes. Malachite green isothiocyanate (MGITC) showed faster cell membrane transport than malachite green (MG).

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Area of Science:

Biophysics
Cell Biology
Pharmacology

Background:

Understanding molecular interactions with cell membranes is crucial for drug delivery.
The plasma membrane's properties influence the uptake and transport of therapeutic agents.
Non-small cell lung cancer (H596) cells provide a relevant model for studying drug-membrane dynamics.

Purpose of the Study:

To investigate the real-time physicochemical interactions of cationic molecules with human lung cancer cell membranes.
To compare the adsorption and transport kinetics of malachite green (MG) and malachite green isothiocyanate (MGITC).
To assess the impact of cell integrity on molecular translocation dynamics.

Main Methods:

Time-resolved second harmonic generation (SHG) microscopy was employed.
Studies were conducted on both living and fixed H596 cells.
Molecular adsorption and transport of MG and MGITC were monitored in real time.

Main Results:

MGITC exhibited stronger adsorption and faster transport kinetics than MG.
Dipole-dipole interactions contributed to MGITC's enhanced membrane interactions.
MGITC showed faster kinetics and greater transport rate variability in living cells compared to fixed cells.

Conclusions:

Electrostatic interactions and chemical functional groups significantly influence molecular transport across cell membranes.
Cell membrane integrity plays a critical role in the dynamics of molecular translocation.
Findings provide insights into the mechanisms governing drug-membrane interactions for potential therapeutic applications.