Related Experiment Video
Updated: May 22, 2025
07:34
Induction of Intestinal Inflammation by Adoptive Transfer of CBir1 TCR Transgenic CD4+ T Cells to Immunodeficient Mice
Published on: December 16, 2021
2.4K
TXM-CB13 Improves the Intestinal Mucosal Barrier and Alleviates Colitis by Inhibiting the ROS/TXNIP/TRX/NLRP3 and
Ruijie Cao1, Jinhui Zhou1, Jiale Liu1
1Department of Basic Medicine, Medical College, Shaoxing University, Shaoxing, China.
Inflammation
|March 14, 2025
Summary
The thioredoxin-mimetic peptide CB13 (TXM-CB13) alleviates inflammatory bowel disease (IBD) symptoms by inhibiting NLRP3 inflammasome activation via TLR4 and ROS pathways.
Area of Science:
- Immunology
- Gastroenterology
- Molecular Biology
Background:
- Inflammasome activation, specifically NLRP3 and NLRP1, is critical in inflammatory bowel disease (IBD) pathogenesis.
- Thioredoxin-mimetic peptide CB13 (TXM-CB13) possesses known antioxidative stress and anti-inflammatory properties.
Purpose of the Study:
- To investigate the protective effects of TXM-CB13 in dextran sulfate sodium (DSS)-induced colitis models.
- To elucidate the molecular mechanisms underlying TXM-CB13's action on inflammasome activation in macrophages.
Main Methods:
- DSS-induced colitis model in vivo and lipopolysaccharide (LPS)-induced inflammasome activation in RAW264.7 macrophages in vitro.
- Analysis of inflammasome components (NLRP3, ASC, Caspase-1), inflammatory cytokines (IL-1β), signaling pathways (TLR4, MyD88, NF-κB), and intestinal barrier proteins (Occludin, ZO-1) using Western blot and immunohistochemistry.
- Assessment of reactive oxygen species (ROS) and thioredoxin-interacting protein (TXNIP) dissociation from thioredoxin (TRX).
Main Results:
- TXM-CB13 significantly alleviated DSS-induced colitis symptoms.
- TXM-CB13 suppressed NLRP3, Mlck, and IL-1β levels in colonic tissues and increased intestinal barrier proteins.
- In vitro, TXM-CB13 inhibited LPS-induced TLR4/MyD88/NF-κB signaling, reducing NLRP3 inflammasome activation and ROS-mediated TXNIP/TRX dissociation.
Conclusions:
- TXM-CB13 demonstrates therapeutic potential for IBD by mitigating inflammation and enhancing intestinal barrier function.
- TXM-CB13 acts through dual pathways: modulating TLR4/MyD88/NF-κB/NLRP3 and ROS/TXNIP/TRX/NLRP3 signaling cascades.
More Related Videos
08:58Analyzing Beneficial Effects of Nutritional Supplements on Intestinal Epithelial Barrier Functions During Experimental Colitis
Published on: January 5, 2017
12.2K
08:37Induction of Murine Intestinal Inflammation by Adoptive Transfer of Effector CD4+CD45RBhigh T Cells into Immunodeficient Mice
Published on: April 21, 2015
16.4K
Related Concept Videos
01:24Drugs for Treatment of Crohn's Disease in IBD Using Biologic Agents: Anti-TNF
107
Tumor Necrosis Factor (TNF), a proinflammatory cytokine, contributes significantly to the inflammation seen in Crohn's disease. It exists as soluble TNF and membrane-bound TNF, with actions mediated through TNF receptors (TNFR). TNFR activation leads to the release of proinflammatory cytokines, T-cell activation, collagen production, and leukocyte migration, all contributing to inflammation in Crohn's disease. Anti-TNF monoclonal antibodies, namely infliximab (Remicade), adalimumab...
107
01:30Inflammatory Bowel Disease II: Crohn's Disease
151
Introduction
Inflammatory bowel disease, commonly known as IBD, refers to a collection of disorders that lead to persistent inflammation of the gastrointestinal tract. The two types of IBD are ulcerative colitis, which impacts the colon, and Crohn's disease, which can involve any part of the gastrointestinal segment.
Crohn's disease
Crohn's disease is a chronic, systemic inflammatory bowel disease (IBD) that predominantly affects the gastrointestinal tract. It is marked by...
Inflammatory bowel disease, commonly known as IBD, refers to a collection of disorders that lead to persistent inflammation of the gastrointestinal tract. The two types of IBD are ulcerative colitis, which impacts the colon, and Crohn's disease, which can involve any part of the gastrointestinal segment.
Crohn's disease
Crohn's disease is a chronic, systemic inflammatory bowel disease (IBD) that predominantly affects the gastrointestinal tract. It is marked by...
151
01:27Inflammatory Bowel Disease I: Ulcerative Colitis
106
Introduction
Inflammatory bowel disease, or IBD, encompasses a group of disorders characterized by chronic inflammation or ulceration of the gastrointestinal tract.
Risk Factors
The exact cause of IBD remains unclear, although it is believed to be due to a mix of genetic, environmental, microbial, and immune factors. Genetic factors are significant in determining susceptibility to IBD, with family history being a critical risk factor. Individuals with a first-degree relative who has IBD are at...
Inflammatory bowel disease, or IBD, encompasses a group of disorders characterized by chronic inflammation or ulceration of the gastrointestinal tract.
Risk Factors
The exact cause of IBD remains unclear, although it is believed to be due to a mix of genetic, environmental, microbial, and immune factors. Genetic factors are significant in determining susceptibility to IBD, with family history being a critical risk factor. Individuals with a first-degree relative who has IBD are at...
106
01:17Chronic Bowel Disorders: Introduction
375
Chronic bowel diseases are a group of long-term conditions affecting the digestive tract, characterized by inflammation and damage to the gut lining. These conditions primarily include irritable bowel syndrome and inflammatory bowel disease.
Irritable Bowel Syndrome (IBS) is a common disorder affecting the gastrointestinal tract. The distinctive feature is recurrent abdominal pain associated with altered bowel movements, manifesting as constipation, diarrhea, or fluctuating between both. The...
Irritable Bowel Syndrome (IBS) is a common disorder affecting the gastrointestinal tract. The distinctive feature is recurrent abdominal pain associated with altered bowel movements, manifesting as constipation, diarrhea, or fluctuating between both. The...
375
01:24T Cell Types and Functions
740
When T cells with CD4 markers are activated, they give rise to two types of effector cells: helper T cells and regulatory T cells. Meanwhile, T cells with CD8 markers differentiate into effector cytotoxic T cells. The differentiation of CD4 T cells into helper T cell subsets, such as Th1, Th2, and Th17 cells, is dependent on the antigen type, antigen-presenting cell, and regulatory cytokines.
Th1 cells stimulate dendritic cells to express necessary co-stimulatory molecules on their surfaces for...
Th1 cells stimulate dendritic cells to express necessary co-stimulatory molecules on their surfaces for...
740
01:17Irritable Bowel Syndrome I: Introduction
213
Irritable Bowel Syndrome (IBS) is characterized by functional disturbances in the gastrointestinal system, presenting a cluster of symptoms without evident structural or biochemical abnormalities. It primarily affects the large intestine and may cause abdominal pain, bloating, excessive gas, diarrhea, constipation, or both.
IBS is a chronic condition that can persist over a long period or recur frequently.
The pathogenesis of IBS involves a complex interplay of the following factors:
Altered...
IBS is a chronic condition that can persist over a long period or recur frequently.
The pathogenesis of IBS involves a complex interplay of the following factors:
Altered...
213