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Updated: May 11, 2026

Combining Analysis of DNA in a Crude Virion Extraction with the Analysis of RNA from Infected Leaves to Discover New Virus Genomes
Published on: July 27, 2018
Characterization of a betaendornavirus isolated from the edible fungus Morchella sextelata
Ruiling Lyu1,2, Junlin Chen2, Qing Tang1,3
1State Key Laboratory of Agricultural Microbiology, Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan, Hubei, 430070, China.
Abstract:
A positive-sense single-stranded RNA mycovirus was isolated from Morchella sextelata strain ZY-1 and designated as "Morchella sextelata endornavirus 1" (MsEV1). The complete genome of MsEV1 was found to be 16,589 nucleotides (nt) in length and to contain a single large open reading frame (ORF) encoding the viral proteins PRK00409, transmethylase, DEXDc, helicase, and RdRP in a polyprotein of 5,477 amino acids. Phylogenetic analysis based on RdRP nucleotide sequences revealed that this endornavirus is a member of the genus Betaendornavirus in the family Endornaviridae.
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Fungal Group Zygomycota
Fungal Phylum Microsporidia
Fungal Phylum Basidiomycota

