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Related Experiment Video

Updated: May 22, 2025

Growth of Human and Sheep Corneal Endothelial Cell Layers on Biomaterial Membranes

Growth of Human and Sheep Corneal Endothelial Cell Layers on Biomaterial Membranes

Published on: February 6, 2020

Decellularization of camel corneas for tissue engineering applications.

Ali Doostmohammadi^1,2, Athar Talebi¹, Fateme Naghibi²

¹Nervous System Stem Cells Research Center, Semnan University of Medical Sciences, Semnan, Iran.

International Ophthalmology

|March 14, 2025

Summary

Camel cornea (CC) decellularization using sodium dodecyl sulfate (SDS) or formic acid (FA) methods preserves its structure. CC shows high protein similarity to human cornea, making it a promising scaffold for tissue engineering.

Keywords:

Cornea Decellularized scaffold Organic acid Tissue engineering

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Corneal Tissue Engineering: An In Vitro Model of the Stromal-nerve Interactions of the Human Cornea

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Corneal Tissue Engineering: An In Vitro Model of the Stromal-nerve Interactions of the Human Cornea

Corneal Tissue Engineering: An In Vitro Model of the Stromal-nerve Interactions of the Human Cornea

Published on: January 24, 2018

Area of Science:

Biomaterials Science
Ophthalmology
Tissue Engineering

Background:

Camel cornea (CC) shares collagen fiber diameter similarities with human cornea.
CC's structural properties suggest potential for tissue engineering applications.

Purpose of the Study:

To evaluate decellularization methods on CC.
To compare protein content between CC and human cornea.
To highlight CC's potential for human tissue engineering.

Main Methods:

Decellularization of CC using SDS- and FA-based protocols.
Analysis of decellularized CC (dCC) for histology, cell viability, collagen integrity, and ultrastructure.
Comparison of amino acid composition between CC and human cornea proteins.

Main Results:

Successful decellularization achieved with SDS and FA methods.
Increased cell viability on dCC scaffolds, particularly with 0.5% SDS-Tris treatment.
Effective preservation of collagen fibers by OrgA and 0.5% SDS-Tris protocols.
Notable resemblance in amino acid composition between CC and human cornea.

Conclusions:

CC can be effectively decellularized using SDS and FA methods, preserving key tissue characteristics.
FA treatment requires caution due to potential bacterial contamination.
High amino acid similarity supports CC's use as a scaffold for human tissue engineering.