Understanding your Biases in Collecting Organismal VOCs

Volatile organic compounds (VOCs) play a fundamental role in organismal interactions, facilitating intra- and interspecific communications. Accurate collection and analysis of VOCs are essential for understanding these interactions, but the choice of collection methods and adsorbent materials can introduce biases. This study investigates the variability and recovery yield in VOC collection using various adsorbents and thin-film solid-phase microextraction (TF-SPME). We compared the performance of nine adsorbents and TF-SPME in capturing a standard VOC mixture and VOCs from rosemary plants. Results show significant differences in efficiency among adsorbents, with Porapak^® P generally providing the best recovery for most compounds. TF-SPME exhibited higher sensitivity and detected a broader range of volatiles compared to adsorbents, though qualitative profiles varied. Our findings underscore the importance of empirical testing in adsorbent selection and highlight the inherent biases in VOC collection methods. These insights aim to guide and empower researchers in making informed decisions regarding experimental design and data interpretation to achieve more accurate and reliable VOC results in chemical ecology studies.