Importance of the preanalytical phase: Elevation of protein levels in cerebrospinal fluid samples due to...

Background: Biomarkers in cerebrospinal fluid (CSF) are crucial for diagnosing, monitoring, and prognosing neurological disorders.Purpose: This study evaluates the impact of preanalytical variables, particularly container choice, on CSF protein measurements.Analysis: Using 30 CSF samples, we compared sterile, additive-free tubes and lithium heparin tubes without separator gel.Results: Protein levels were significantly elevated higher in heparin tubes (mean difference: 230.71 mg/dL, P < .001).Conclusions: This overestimation underscores the necessity of adhering to preanalytical protocols to avoid erroneous clinical interpretations and ensure accurate diagnostic outcomes.