Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Microbial Biosensors

Microbial Biosensors

Microbial biosensors are analytical devices that utilize living microbes to detect specific substances through measurable signals. These devices consist of two main components: biosensing organisms and signal-transducing elements. Biosensing organisms, such as Escherichia coli or Saccharomyces cerevisiae, are typically housed in multiwell plates connected to transducers, enabling rapid, real-time detection of target analytes.Signal Generation MechanismWhen a target analyte—such as...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Flexible Carbon Nanotube Complementary Metal-Oxide-Semiconductor Integrated Circuits with Ultrastrong Radiation Resistance.

Nano letters·2026

Same author

Modular Functionalized Gates for Field-Effect Transistor Biosensors Enabling Reliable Detection of Trace miRNAs.

ACS nano·2026

Same author

Electrostatically Reconfigurable Gas Sensing Units and Arrays Based on Carbon Nanotube Transistors.

ACS applied materials & interfaces·2026

Same author

Collective quantum coherence and subband redistribution in artificially assembled nanotube arrays.

National science review·2026

Same author

Danggui Shaoyao San ameliorates neuroinflammation in a D-galactose-induced Alzheimer's disease rat model by suppressing the JAK2/STAT3 pathway and modulating Th17/Treg -related immune dysregulation.

Frontiers in cell and developmental biology·2026

Same author

Magnetic molecularly imprinted polymer-assisted target enrichment for the fluorescence detection of chloramphenicol in water.

The Analyst·2026

Search research articles

Related Experiment Video

Updated: May 1, 2026

Fabrication of Electrochemical-DNA Biosensors for the Reagentless Detection of Nucleic Acids, Proteins and Small Molecules

Fabrication of Electrochemical-DNA Biosensors for the Reagentless Detection of Nucleic Acids, Proteins and Small Molecules

Published on: June 1, 2011

Small-Molecule Self-Assembly Strategy for Ultrafast, Sensitive, and Portable Multiplexed Antibiotics Detection by

Jiajun Tong¹, Mengmeng Xiao^1,2, Kemin Wang¹

¹Hunan Institute of Advanced Sensing and Information Technology, Hunan Provincial Key Laboratory of Smart Carbon Materials and Advanced Sensing, Xiangtan University, Xiangtan, Hunan 411105, China.

|March 14, 2025

Summary

This summary is machine-generated.

A novel carbon nanotube field-effect transistor (CNT-FET) biosensor array offers rapid, sensitive detection of multiple antibiotics at femtomolar levels. This technology is crucial for infant health, addressing risks from antibiotic coexposure and drug resistance.

Keywords:

antibiotics carbon nanotube field-effect transistor biosensor arrays portable detection self-assembled monolayer

More Related Videos

Microfluidic On-chip Capture-cycloaddition Reaction to Reversibly Immobilize Small Molecules or Multi-component Structures for Biosensor Applications

Microfluidic On-chip Capture-cycloaddition Reaction to Reversibly Immobilize Small Molecules or Multi-component Structures for Biosensor Applications

Published on: September 23, 2013

Ultrasensitive Detection of Biomarkers by Using a Molecular Imprinting Based Capacitive Biosensor

Ultrasensitive Detection of Biomarkers by Using a Molecular Imprinting Based Capacitive Biosensor

Published on: February 16, 2018

Related Experiment Videos

Last Updated: May 1, 2026

Fabrication of Electrochemical-DNA Biosensors for the Reagentless Detection of Nucleic Acids, Proteins and Small Molecules

Fabrication of Electrochemical-DNA Biosensors for the Reagentless Detection of Nucleic Acids, Proteins and Small Molecules

Published on: June 1, 2011

Microfluidic On-chip Capture-cycloaddition Reaction to Reversibly Immobilize Small Molecules or Multi-component Structures for Biosensor Applications

Microfluidic On-chip Capture-cycloaddition Reaction to Reversibly Immobilize Small Molecules or Multi-component Structures for Biosensor Applications

Published on: September 23, 2013

Ultrasensitive Detection of Biomarkers by Using a Molecular Imprinting Based Capacitive Biosensor

Ultrasensitive Detection of Biomarkers by Using a Molecular Imprinting Based Capacitive Biosensor

Published on: February 16, 2018

Area of Science:

Biosensor technology
Nanomaterials science
Analytical chemistry

Background:

Urgent need for portable, sensitive, and accurate methods to detect multiple antibiotics, especially for infants, to combat drug resistance.
Field-effect transistor (FET) biosensors show promise but face sensitivity and selectivity challenges in complex samples.
Existing methods like ELISA may lack the required sensitivity or speed for real-time monitoring.

Purpose of the Study:

To develop a portable biosensor array for simultaneous detection and quantification of multiple antibiotics at low concentrations.
To enhance the sensitivity and selectivity of carbon nanotube FET (CNT-FET) biosensors for practical applications.
To create a system superior to ELISA for antibiotic detection in complex matrices like milk.

Main Methods:

Utilized a small-molecule coating strategy on CNT-FET biosensor arrays to reduce nonspecific adsorption and Debye shielding.
Incorporated aptamers for specific antibiotic recognition via inkjet printing.
Developed a portable detection system integrating the enhanced FET biosensor chip.

Main Results:

Achieved an ultrafast response time of 100 seconds for antibiotic detection.
Demonstrated high sensitivity at the femtomolar level for simultaneous detection of kanamycin, oxytetracycline, and sulfaquinoxaline.
Exhibited a wide linear range (femtomolar to nanomolar) and high accuracy (91.1-107.5% recovery rate).

Conclusions:

The developed CNT-FET biosensor array enables accurate quantification of multiple antibiotics at extremely low concentrations in milk.
This technology surpasses ELISA in performance and offers potential for detecting other biomarkers like toxins and hormones.
The portable system addresses critical needs in monitoring antibiotic exposure and mitigating health risks.