Related Experiment Video
Updated: May 22, 2025

Analysis of the Lipid Composition of Mycobacteria by Thin Layer Chromatography
Published on: April 16, 2021
Correlation of Cytomorphology Pattern in Tuberculous Lymphadenitis with Microbiological Studies
Yasmeen Khatib1, Vaz G Sheldon2, Jayashri Chaudhari3
1Professor, Department of Pathology, Hinduhrudaysamrat Balasaheb Thackarey Medical College and Dr Rustom Narsi Cooper Municipal General Hospital, Mumbai, Maharashtra, India.
Diagnosing tuberculous lymphadenitis requires combining fine needle aspiration cytology with microbiological tests like Cartridge-Based Nucleic Acid Amplification Testing (CBNAAT) and Mycobacterium Growth Indicator Tube Culture (MGIT) for improved accuracy.
Area of Science:
- Medical Microbiology
- Cytopathology
- Infectious Diseases
Background:
- Fine needle aspiration cytology (FNAC) is a primary diagnostic tool for suspected tuberculous lymphadenitis.
- Microbiological tests are crucial for diagnosis confirmation and drug resistance but often have low individual sensitivity.
Purpose of the Study:
- To analyze cytomorphological patterns in tuberculous lymphadenitis.
- To compare the diagnostic performance of Ziehl-Neelsen (ZN) stain, Auramine-Rhodamine (AR) stain, Mycobacterium Growth Indicator Tube Culture (MGIT), and Cartridge-Based Nucleic Acid Amplification Testing (CBNAAT).
Main Methods:
- A prospective study involving 100 clinically suspected cases of tuberculous lymphadenitis undergoing FNAC.
- Analysis of cytological material for cytomorphology, ZN stain, AR stain, MGIT, and CBNAAT.
Main Results:
- Out of 100 cases, 94 confirmed tuberculous lymphadenitis with diverse cytomorphological patterns.
- Microbiological test positivity rates: AR stain (17.02%), ZN stain (7%), CBNAAT (45.74%), MGIT (59.57%).
- Combined cytomorphology with microbiological tests achieved 94.7% sensitivity.
Conclusions:
- Individual microbiological tests for tuberculous lymphadenitis show limited sensitivity.
- A combination of cytomorphology with CBNAAT and MGIT is recommended for optimal diagnosis and management.
More Related Videos
09:58Spatial Quantification of Drugs in Pulmonary Tuberculosis Lesions by Laser Capture Microdissection Liquid Chromatography Mass Spectrometry LCM-LC/MS
Published on: April 18, 2018
07:42A Novel Microdissection Approach to Recovering Mycobacterium tuberculosis Specific Transcripts from Formalin Fixed Paraffin Embedded Lung Granulomas
Published on: June 5, 2014
Related Concept Videos
Pulmonary Tuberculosis IV
Several diagnostic approaches are used to detect TB. The conventional method is the Tuberculin Skin Test (TST), also known as the Mantoux test. However, this method has...
Pulmonary Tuberculosis III
The first classification is based on the development of the disease, and it includes the following categories:
Sputum Studies I: Gram Stain, cytology, and Acid-fast smear and culture
Gram Stain
The Gram Stain is an integral part of sputum studies. It involves the staining of sputum, which permits...
Pulmonary Tuberculosis II
Here is a detailed explanation of its pathophysiology:
Transmission: The process begins when a person inhales droplet nuclei containing M. tuberculosis. These are typically released into the air when an individual with pulmonary or...
Pulmonary Tuberculosis I
Causative Organism
The primary infectious agent causing tuberculosis is Mycobacterium tuberculosis, a slow-growing, acid-fast, aerobic rod that exhibits sensitivity to heat and ultraviolet light. Instances of Mycobacterium bovis and Mycobacterium avium contributing to the development of TB infection are rare.
Mode of...