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Updated: May 22, 2025

In Vitro Selection of Aptamers to Differentiate Infectious from Non-Infectious Viruses

In Vitro Selection of Aptamers to Differentiate Infectious from Non-Infectious Viruses

Published on: September 7, 2022

Droplet digital PCR-based single aptamer selection.

Zerui Wu¹, Wanjun Yao², Jinyu Chen¹

¹Department of Thermal Science and Energy Engineering, University of Science and Technology of China, Hefei, 230027, China.

|March 15, 2025

Summary

This summary is machine-generated.

This study introduces a novel single-step method for aptamer discovery, bypassing traditional SELEX. This digital selection process rapidly identifies target aptamers, enhancing aptamer-based applications.

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Last Updated: May 22, 2025

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Area of Science:

Biotechnology
Molecular Biology
Chemical Biology

Background:

Aptamers offer a potent alternative to antibodies for diagnostics and therapeutics.
The traditional Systematic Evolution of Ligands by Exponential Enrichment (SELEX) process is time-consuming, costly, and stochastic.

Purpose of the Study:

To develop a rapid, single-step method for direct aptamer acquisition.
To bypass the limitations of traditional SELEX and sequencing.

Main Methods:

Aptamers are encapsulated into droplets with target molecules and amplified.
Fluorescence indicates aptamer-target binding affinity.
Digital sorting of fluorescing droplets enables direct aptamer identification.

Main Results:

The developed method allows for the direct acquisition of target aptamers in a single step.
This digital selection process reduces stochastic events and costs associated with traditional sequencing.
Proof-of-concept findings demonstrate the strategy's effectiveness in yielding desired aptamers.

Conclusions:

This straightforward strategy significantly enhances aptamer discovery.
It accelerates the exploration of aptamer biology and the development of aptamer-based applications.