Chitosan stabilized platinum nanoparticles: In vitro and in vivo screening for analgesic and anti-inflammatory...

In this interdisciplinary research work, the chitosan stabilized platinum nanoparticles are synthesized through the wet chemical method, and the structural, surface morphological, and optical characterizations are done using X-ray crystallography, Raman spectroscopy, transmission electron microscopy, etc. The samples were tested in in vitro trials namely egg albumin denaturation assay and DPPH radical scavenging assays and showed significantly lower effective concentrations (EC₅₀) such as 5.44 μg/ml and 8.068 μg/ml respectively. The in vitro experiments were followed by in vivo animal model for analgesic and anti-inflammatory behaviour at two doses of 25 mg/kg and 50 mg/kg utilizing the hot plate method and the carrageenan-induced paw edema model respectively. The in vivo hot plate model for analgesic effect demonstrated that the chitosan stabilized platinum nanoparticles perform exceptionally well and show >90 % analgesia (p < 0.01) by extending the reaction time in the hot plate method-indicating better analgesia. Carrageenan-induced paw edema model demonstrated the exceptional anti-inflammatory ability of chitosan-stabilized platinum nanoparticles. Despite being given at a comparatively lower dosage, chitosan stabilized platinum nanoparticles showed a considerable decrease in paw volume (40-45 % edema inhibition) by the third hour of the anti-inflammatory experimentation (p < 0.01) outperforming the standard drug aspirin given at 100 mg/kg.