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Cryo-electron Microscopy

Cryo-electron Microscopy

Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...

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Cryosectioning of Contiguous Regions of a Single Mouse Skeletal Muscle for Gene Expression and Histological Analyses

Cryosectioning of Contiguous Regions of a Single Mouse Skeletal Muscle for Gene Expression and Histological Analyses

Published on: December 12, 2016

Transcriptomics of cryopreserved cells.

Y O Ciftci¹, E Kaya²

¹Molecular Biology and Genetics Department; Smart Agriculture Research and Application Center; Central Research Laboratory (GTU-MAR), Faculty of Basic Sciences, Gebze Technical University, Kocaeli, Turkiye. ozden@gtu.edu.tr.

|March 16, 2025

Summary

This summary is machine-generated.

Transcriptomics, the study of gene expression, reveals how cells survive cryopreservation. This approach helps identify genes and pathways crucial for cell viability and understanding cryodamage in plants and mammals.

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Area of Science:

Cryobiology
Molecular Biology
Genomics

Background:

Cryopreservation is vital for genetic resource conservation but cellular stress mechanisms remain unclear.
Understanding molecular adjustments during cryopreservation is essential for improving viability.

Purpose of the Study:

To review recent studies integrating cryopreservation with transcriptomics (cryobiomics).
To assess factors optimizing mammalian and plant cell cryopreservation.
To explore transcriptomics' role in understanding cryodamage and cold tolerance.

Main Methods:

Review of scientific literature utilizing transcriptomic techniques.
Analysis of gene expression profiles before and after cryopreservation.
Application of methods like RT-PCR, real-time PCR, and RNA-seq.

Main Results:

Transcriptomics provides a global view of gene expression changes in cryopreserved cells.
Identifies genes, non-coding RNAs, and pathways affecting cell viability and proliferation.
Highlights transcriptomics' utility in understanding cryodamage, developmental disorders, and plant cold tolerance.

Conclusions:

Transcriptomics is a powerful tool for understanding cellular responses to cryopreservation.
It aids in optimizing cryopreservation protocols for various cell types.
Further research using transcriptomics can elucidate cryo-recalcitrance mechanisms in plants.