Related Experiment Video
Updated: May 22, 2025
Induction of Nephrotic Syndrome in Mice by Retrobulbar Injection of Doxorubicin and Prevention of Volume Retention by Sustained Release Aprotinin
Published on: May 6, 2018
Activation ADORA1 protects against sepsis-associated acute kidney injury by inhibiting pyroptosis
Wei He1, Kaixin Pan1, Chenggen Xiao1
1Department of Emergency, Xiangya Hospital, Central South University, Changsha, Hunan, China.
Background:
The ADORA1 is known to provide renoprotection against acute kidney injury. However, the underlying mechanisms remain unclear. The purpose of this study was to investigate whether and how ADORA1 plays a role in renoprotection in sepsis associated acute kidney injury (SA-AKI).
Methods:
Sepsis model was induced by lipopolysaccharide (LPS) in male C57BL/6 mice, 0.9 % NS served as controls. Animals received ADORA1 agonists and antagonist before the LPS. Renal function, histology and pyroptosis markers were assessed, with simultaneous validation by vitro assays.
Results:
The animals treated with ADORA1 agonists exhibited higher survival rates and an improved renal functional recovery, attenuated histological lesions and downgraded pyroptosis. Moreover, which down-regulated the expression of cleaved caspase 11 and GSDMD, while the ADORA1 antagonist group exhibit an oppose results.
Conclusions:
ADORA1 protects against SA-AKI, at least in part, through its inhibitory effects on pyroptosis via the noncanonical inflammasome pathway. If our finding may extrapolated to clinical setting, ADORA1 agonist may serve as a clinical strategy to SA-AKI.
More Related Videos
07:03Standardized Colon Ascendens Stent Peritonitis in Rats - a Simple, Feasible Animal Model to Induce Septic Acute Kidney Injury
Published on: February 15, 2022
07:15Mechanism of Kemeng Fang's Inhibition of Podocyte Apoptosis in Rats with Membranous Nephropathy through the PI3K/AKT Signaling Pathway
Published on: August 23, 2024
