Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Covalently Linked Protein Regulators02:04

Covalently Linked Protein Regulators

6.7K
Proteins can undergo many types of post-translational modifications, often in response to changes in their environment. These modifications play an important role in the function and stability of these proteins. Covalently linked molecules include functional groups, such as methyl, acetyl, and phosphate groups, and also small proteins, such as ubiquitin. There are around 200 different types of covalent regulators that have been identified.
These groups modify specific amino acids in a protein....
6.7K
Drug Metabolism: Phase II Reactions01:14

Drug Metabolism: Phase II Reactions

3.5K
Phase II reactions are essential for the detoxification and elimination of drugs from the body. These reactions involve the conjugation of parent drugs or their phase I metabolites with endogenous molecules, resulting in more hydrophilic drug conjugates. The primary conjugation reactions in this phase are sulfation and glucuronidation. Both sulfation and glucuronidation typically produce biologically inactive metabolites. However, in some cases involving prodrugs, active metabolites may be...
3.5K
Transducer Mechanism: Enzyme-Linked Receptors01:27

Transducer Mechanism: Enzyme-Linked Receptors

2.3K
Enzyme-linked receptors are cell-surface receptors acting as an enzyme or associating with an enzyme intracellularly. They make excellent drug targets. Drugs can bind to the extracellular ligand-binding domain or directly affect their enzymatic domain and alter their activity.
Major types that are helpful drug targets include:
2.3K
The Proteasome02:18

The Proteasome

8.4K
Eukaryotic cells can degrade proteins through several pathways. One of the most important amongst these is the ubiquitin-proteasome pathway. It helps the cell eliminate the misfolded, damaged, or unwarranted cytoplasmic proteins in a highly specific manner.
In this pathway, the target proteins are first tagged with small proteins called ubiquitin. A series of enzymes carry out the ubiquitination of the target proteins - E1 (ubiquitin-activating enzyme), E2 (ubiquitin-conjugating enzyme), and E3...
8.4K
Phase II Reactions: Sulfation and Conjugation with α-Amino Acids01:19

Phase II Reactions: Sulfation and Conjugation with α-Amino Acids

144
Sulfation and α-amino acid conjugation are two critical biotransformation reactions in drug metabolism. Sulfation, a phase II biotransformation reaction, involves adding a polar sulfate group to a drug, enhancing its water solubility and promoting excretion. This process can either co-occur with or occur independently of glucuronidation. Nonmicrosomal sulfotransferase enzymes catalyze the process. The reaction involves 3'-phosphoadenosine-5'-phosphosulfate or PAPS coenzyme...
144
Targeted Cancer Therapies02:57

Targeted Cancer Therapies

7.4K
The targeted cancer therapies, also known as “molecular targeted therapies,” take advantage of the molecular and genetic differences between the cancer cells and the normal cells. It needs a thorough understanding of the cancer cells to develop drugs that can target specific molecular aspects that drive the growth, progression, and spread of cancer cells without affecting the growth and survival of other normal cells in the body.
There are several types of targeted therapies against...
7.4K
  1. Home
  3. Protein Succinylation Mechanisms And Potential Targeted Therapies In Urinary Disease.
  1. Home
  3. Protein Succinylation Mechanisms And Potential Targeted Therapies In Urinary Disease.

Related Experiment Video

Simultaneous Affinity Enrichment of Two Post-Translational Modifications for Quantification and Site Localization
12:11

Simultaneous Affinity Enrichment of Two Post-Translational Modifications for Quantification and Site Localization

Published on: February 27, 2020

6.7K

Protein succinylation mechanisms and potential targeted therapies in urinary disease.

Yuanquan Lou1, Caitao Dong1, Qinhong Jiang1

  • 1Department of Urology, Renmin Hospital of Wuhan University, Wuhan 430060, Hubei Province, People's Republic of China.

Cellular Signalling
|March 16, 2025

View abstract on PubMed

Summary
This summary is machine-generated.

Succinylation, a protein modification, impacts cell metabolism and is implicated in urinary diseases. Targeting succinylation enzymes may offer new therapeutic strategies for these conditions.

Keywords:
Metabolic pathwaysPost-translational modificationSuccinylationTargeted therapiesUrinary disease

More Related Videos

Utilizing a Comprehensive Immunoprecipitation Enrichment System to Identify an Endogenous Post-translational Modification Profile for Target Proteins
08:12

Utilizing a Comprehensive Immunoprecipitation Enrichment System to Identify an Endogenous Post-translational Modification Profile for Target Proteins

Published on: January 8, 2018

11.2K
Evaluation of Substrate Ubiquitylation by E3 Ubiquitin-ligase in Mammalian Cell Lysates
09:47

Evaluation of Substrate Ubiquitylation by E3 Ubiquitin-ligase in Mammalian Cell Lysates

Published on: May 10, 2022

2.4K

Related Experiment Videos

Simultaneous Affinity Enrichment of Two Post-Translational Modifications for Quantification and Site Localization
12:11

Simultaneous Affinity Enrichment of Two Post-Translational Modifications for Quantification and Site Localization

Published on: February 27, 2020

6.7K
Utilizing a Comprehensive Immunoprecipitation Enrichment System to Identify an Endogenous Post-translational Modification Profile for Target Proteins
08:12

Utilizing a Comprehensive Immunoprecipitation Enrichment System to Identify an Endogenous Post-translational Modification Profile for Target Proteins

Published on: January 8, 2018

11.2K
Evaluation of Substrate Ubiquitylation by E3 Ubiquitin-ligase in Mammalian Cell Lysates
09:47

Evaluation of Substrate Ubiquitylation by E3 Ubiquitin-ligase in Mammalian Cell Lysates

Published on: May 10, 2022

2.4K

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Cellular Metabolism

Background:

  • Succinylation is a prevalent post-translational modification occurring in various cellular compartments.
  • It involves the modification of histone and non-histone proteins, regulated by succinyltransferases and desuccinylases like sirtuin 5.
  • Proper regulation of succinylation is crucial for maintaining cellular homeostasis and metabolic balance.

Purpose of the Study:

  • To explore the role of succinylation in the pathogenesis of urinary diseases.
  • To investigate how dysregulated succinylation affects metabolic pathways and gene expression in the context of urinary diseases.
  • To evaluate the potential of targeting succinylation-related enzymes as a therapeutic approach for urinary diseases.

Main Methods:

  • Review of existing literature on protein succinylation, its regulatory mechanisms, and its involvement in metabolic pathways.
  • Analysis of the impact of succinylation on gene expression and enzyme activity in relation to urinary disease progression.
  • Identification of key succinylation-related enzymes (succinyltransferases and desuccinylases) and their roles in cellular metabolism and disease.

    • Main Results:

    • Dysregulated succinylation significantly impacts metabolic pathways, including the TCA cycle, redox balance, and fatty acid metabolism.
    • Succinylation influences disease progression by altering gene expression and modulating enzyme activity, particularly those involved in the Warburg effect.
    • Desuccinylases affect tumor cell energy supply, while succinyltransferases can regulate gene transcription, influencing urinary disease development.

    Conclusions:

    • Succinylation plays a critical role in the development and progression of urinary diseases.
    • Targeting succinylation-related enzymes offers a promising therapeutic avenue for managing urinary diseases by modulating metabolic pathways and gene expression.