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Updated: May 22, 2025

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Hypermethylation of miR-129-2-3p inhibits esophageal cancer proliferation and migration by down-regulating PPP6C

Ailing Tu1, Han Wu2, Junjie Wang2

  • 1Department of Oncology, The First Affiliated Hospital of Soochow University Suzhou, Jiangsu, China.

American Journal of Translational Research
|March 17, 2025
PubMed
Abstract

Insights

Hypermethylated microRNA-129-2-3p (miR-129-2-3p) suppresses esophageal cancer cell growth and migration. This finding suggests miR-129-2-3p is a potential diagnostic biomarker for early esophageal cancer detection.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • MicroRNAs (miRNAs) are key regulators of gene expression, with dysregulation linked to cancer development.
  • Aberrant DNA methylation patterns can influence gene expression and contribute to carcinogenesis.
  • DNA methylation-based biomarkers show promise for early cancer detection.

Purpose of the Study:

  • To investigate the role of miR-129-2-3p in esophageal cancer (EC).
  • To evaluate miR-129-2-3p as a potential diagnostic biomarker for EC.

Main Methods:

  • MethylTarget technology was used to assess miR-129-2-3p methylation in EC tissues.
  • Logistic regression and ROC curve analysis evaluated diagnostic value.
  • Functional assays and luciferase reporter assays identified PPP6C as a direct target.

Main Results:

  • miR-129-2-3p was found to be hypermethylated in EC tissues.
  • Diagnostic analysis yielded an AUC of 0.799, with 88.4% sensitivity and 65.9% specificity.
  • miR-129-2-3p overexpression inhibited EC cell proliferation and migration by downregulating PPP6C.

Conclusions:

  • Hypermethylated miR-129-2-3p inhibits EC cell proliferation and migration via PPP6C downregulation.
  • miR-129-2-3p presents potential as a diagnostic biomarker for esophageal cancer.
Keywords:
MethylationPPP6Cesophageal cancermiR-129-2-3p

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