JoVE - Journal of Visualized Experiments
JoVE Visualize
Contact Us

CCCTC-binding factor regulates splicing factor proline and glutamine-rich to promote malignant growth of osteosarcoma

  • 0Spine Surgery, Affiliated Hospital of Jiangsu University Zhenjiang 212001, Jiangsu, China.
American Journal of Translational Research +

|

March 17, 2025

|

March 17, 2025

View abstract on PubMed

Summary

This summary is machine-generated.

CCCTC-binding factor (CTCF) acts as an oncogene in osteosarcoma by increasing splicing factor proline and glutamine-rich (SFPQ) expression. Targeting the CTCF-SFPQ pathway may offer new osteosarcoma treatment strategies.

Area Of Science

  • Oncology
  • Molecular Biology
  • Gene Regulation

Background

  • CCCTC-binding factor (CTCF) is implicated in various cancers, but its role in osteosarcoma is unclear.
  • Splicing factor proline and glutamine-rich (SFPQ) is recognized as an oncogene in osteosarcoma.
  • Bioinformatic analysis suggests CTCF may regulate SFPQ transcription.

Purpose Of The Study

  • To investigate the function and mechanism of CTCF in osteosarcoma.
  • To explore the regulatory relationship between CTCF and SFPQ in osteosarcoma.

Main Methods

  • Bioinformatic analysis identified potential transcription factors for SFPQ.
  • Quantitative real-time PCR (qRT-PCR) and western blotting (WB) assessed CTCF expression.
  • Cell function assays, dual-luciferase reporter assays, and rescue experiments evaluated CTCF and SFPQ effects.

Main Results

  • CTCF and SFPQ showed a moderate correlation; CTCF was significantly expressed in osteosarcoma.
  • CTCF overexpression boosted osteosarcoma cell proliferation, migration, and invasion.
  • CTCF directly upregulated SFPQ by binding to its promoter; SFPQ knockdown counteracted CTCF's oncogenic effects.

Conclusions

  • CTCF promotes osteosarcoma malignancy by upregulating SFPQ, functioning as an oncogene.
  • The CTCF-SFPQ axis presents a potential therapeutic target for osteosarcoma treatment.

Keywords:

Related Concept Videos

RNA Splicing 01:32

55.9K

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...

Alternative RNA Splicing 02:18

20.9K

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...

mTOR Signaling and Cancer Progression 03:03

3.7K

The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...

TGF - β Signaling Pathway 01:16

7.2K

The TGF-β signaling pathway regulates cell growth, differentiation, adhesion, motility, and development. TGF-β ligands that induce TGF-β signaling are synthesized in their latent form. Several proteases or cell surface receptors such as integrins act upon the latent form, releasing the active ligand. There are three types of mammalian TGF-βs: (TGF-β1, TGF-β2, and TGF-β3) that bind as homodimers or heterodimers to TGF-β receptors. The TGF-β receptors...

Abnormal Proliferation 02:23

4.4K

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...

Cancer-Critical Genes II: Tumor Suppressor Genes 01:05

7.3K

Genes usually encode proteins necessary for the proper functioning of a healthy cell. Mutations can often cause changes to the gene expression pattern, thereby altering the phenotype.
When the function of certain critical genes, especially those involved in cell cycle regulation and cell growth signaling cascades, gets disrupted, it upsets the cell cycle progression. Such cells with unchecked cell cycles start proliferating uncontrollably and eventually develop into tumors.
Such genes that act...