Application of real-time PCR for analysis canine meat (Canis lupus familiaris) in goat's satay for halal authentication study
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View abstract on PubMed
Summary
This summary is machine-generated.A new real-time PCR method accurately detects canine DNA in food for halal authentication. This species-specific primer ensures food safety and integrity, preventing the illegal use of canine meat in products.
Area Of Science
- Food Science
- Molecular Biology
- Genetics
Background
- Canine meat (CM) is non-halal and prohibited for Muslims.
- CM is often used as an adulterant in halal products like satay and meatballs due to lower cost than beef.
- Economic fraud and religious non-compliance are concerns.
Purpose Of The Study
- To develop a novel, species-specific primer for canine DNA analysis.
- To combine this primer with real-time polymerase chain reaction (PCR) for halal authentication.
- To ensure accurate identification of canine DNA in food products.
Main Methods
- Designed a species-specific primer targeting the D-loop region of mitochondrial DNA.
- Validated primer performance: specificity, amplification efficiency (E), sensitivity, repeatability, and linearity.
- Utilized real-time PCR (RT-PCR) for DNA quantification and analysis.
Main Results
- The primer demonstrated high specificity for canine DNA, with an annealing temperature of 57.8°C.
- RT-PCR achieved excellent amplification efficiency (109.7%) and linearity (R²=0.999).
- The method's limit of detection was 31.25 pg/µl, with 2% relative standard deviation, and successfully detected canine DNA in food samples, with no canine DNA found in goat satay.
Conclusions
- RT-PCR with the novel D-loop primer is a specific and accurate tool for canine meat identification.
- This method is effective for halal authentication, ensuring food product integrity.
- The study successfully identified and quantified canine DNA in food, confirming the absence in tested goat satay.
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