Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

High-Performance Liquid Chromatography: Elution Process01:05

High-Performance Liquid Chromatography: Elution Process

373
In High-Performance Liquid Chromatography (HPLC), the elution process is critical to the separation of analytes and the quality of chromatographic results. Elution describes how compounds move through the column and separate based on their interactions with the mobile and stationary phases. This process determines the resolution, peak shape, and retention times in the chromatogram, which are essential for identifying and quantifying components in complex mixtures. Understanding the elution...
373
Principles Of Column Chromatography01:13

Principles Of Column Chromatography

6.6K
The chromatography technique was first invented in 1901 by Michael S. Tswett, a Russian botanist, to separate plant pigments using organic solvents. Further, in 1941, Archer John Porter Martin and R. L. M. Synge modified the technique by packing silica gel into a column. A mixture of amino acids was then separated on the packed column using chloroform and water mixture as the mobile phase. This was the first report on column chromatography. At present, column chromatography is a widely used...
6.6K
High-Performance Liquid Chromatography: Instrumentation00:57

High-Performance Liquid Chromatography: Instrumentation

757
High-performance liquid chromatography, or HPLC, is an analytical technique that separates liquid samples under high pressures. An HPLC instrument consists of glass bottles for storing solvents called mobile phase reservoirs. HPLC-grade solvents are used to maintain high purity, and the dissolved gases are removed using a degasser, such as a vacuum pumping system or sparging with helium. The solvents are then pumped into the analytical column using a screw-driven syringe or reciprocating pumps.
757
High-Performance Liquid Chromatography: Introduction01:11

High-Performance Liquid Chromatography: Introduction

847
High-performance liquid chromatography(HPLC), formerly referred to as High-pressure liquid chromatography, is a powerful technique used to separate, identify, and quantify components in complex mixtures. The term "high pressure" refers to using high pressure to push the liquid mobile phase through the tightly packed columns.
In HPLC, two phases play a critical role in the separation process:
847
Gas Chromatography: Types of Columns and Stationary Phases01:17

Gas Chromatography: Types of Columns and Stationary Phases

412
Gas chromatography (GC) relies on stationary phases to separate and analyze components in a sample. There are two main types of stationary phases: liquid and solid. Liquid stationary phases are non-volatile, thermally stable, and chemically inert liquids coated onto the column. Solid stationary phases are particles of adsorbent material, such as silica gel or molecular sieves.
For an analyte to remain on the column for a sufficient amount of time, it must exhibit some level of compatibility (or...
412
Chromatographic Methods: Terminology01:18

Chromatographic Methods: Terminology

906
Chromatography is an analytical technique widely used in fields such as chemistry, biology, environmental science, and pharmaceuticals to separate the components of a mixture and identify substances between them. The process of chromatography is based on the interactions between two distinct phases: the stationary phase and the mobile phase. The stationary phase is fixed in place by a supporting material, while the mobile phase moves over it, carrying the solutes. As the mobile phase travels,...
906

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Graphical Visualization Approach for Two-Dimensional Liquid Chromatography with Parallel Column Arrays.

ACS measurement science au·2026
Same author

Quantification of residual protein from biocatalysts employed in the synthesis of pharmaceutical drug substances using reversed-phase HPLC.

Journal of chromatography. A·2026
Same author

A rapid reversed-phase liquid chromatography method for the determination of individual monoclonal antibody content in a co-formulated drug product.

Journal of chromatography. A·2025
Same author

Development of a Direct Sampling Module for Compact Capillary Liquid Chromatography.

Journal of separation science·2025
Same author

Characterization of M11-like and OC125-like Monoclonal Antibody Binding to CA125 Tandem Repeats.

Biosensors·2025
Same author

The ACS Division of Analytical Chemistry Awards Program: Honoring Our Recent Awardees.

Analytical chemistry·2025
Same journal

Quantification of Caffeoylquinic Acids and Triterpenes as Targeted Bioactive Compounds of <i>Centella asiatica</i> in Extracts and Formulations by Liquid Chromatography Mass Spectrometry.

Journal of chromatography open·2023
Same journal

Quantification of N, N' N"-triethylenethiophosphoramide, N, N"-triethylenephosphoramide, cyclophosphamide, and 4-hydroxy-cyclophosphamide in microvolume human plasma to support neonatal and pediatric drug studies.

Journal of chromatography open·2022
Same journal

Determination of unbound piperaquine in human plasma by ultra-high performance liquid chromatography tandem mass spectrometry.

Journal of chromatography open·2022
See all related articles
  1. Home
  3. Considerations For Method Development And Method Translation In Capillary Liquid Chromatography: A Tutorial.
  1. Home
  3. Considerations For Method Development And Method Translation In Capillary Liquid Chromatography: A Tutorial.

Related Experiment Video

Liquid Chromatography Coupled to Refractive Index or Mass Spectrometric Detection for Metabolite Profiling in Lysate-based Cell-free Systems
14:42

Liquid Chromatography Coupled to Refractive Index or Mass Spectrometric Detection for Metabolite Profiling in Lysate-based Cell-free Systems

Published on: September 23, 2021

4.7K

Considerations for Method Development and Method Translation in Capillary Liquid Chromatography: A Tutorial.

Eliza K Hanson1, Samuel W Foster1, Christopher Piccolo1

  • 1Department of Chemistry & Biochemistry, Rowan University, Glassboro, NJ 08028.

Journal of Chromatography Open
|March 17, 2025

View abstract on PubMed

Summary
This summary is machine-generated.

This tutorial guides analytical chemists in scaling down High-Performance Liquid Chromatography (HPLC) methods to capillary LC. It covers essential considerations for successful method translation, focusing on columns with inner diameters less than 0.3 mm.

Keywords:
Capillary LC ColumnsCapillary Liquid ChromatographyInstrumentationMethod Development

More Related Videos

Untargeted Metabolomics from Biological Sources Using Ultraperformance Liquid Chromatography-High Resolution Mass Spectrometry UPLC-HRMS
11:00

Untargeted Metabolomics from Biological Sources Using Ultraperformance Liquid Chromatography-High Resolution Mass Spectrometry UPLC-HRMS

Published on: May 20, 2013

22.4K
Author Spotlight: Optimizing Affinity Chromatography for His-Tagged FEN1 Protein
07:19

Author Spotlight: Optimizing Affinity Chromatography for His-Tagged FEN1 Protein

Published on: April 26, 2024

1.5K

Related Experiment Videos

Liquid Chromatography Coupled to Refractive Index or Mass Spectrometric Detection for Metabolite Profiling in Lysate-based Cell-free Systems
14:42

Liquid Chromatography Coupled to Refractive Index or Mass Spectrometric Detection for Metabolite Profiling in Lysate-based Cell-free Systems

Published on: September 23, 2021

4.7K
Untargeted Metabolomics from Biological Sources Using Ultraperformance Liquid Chromatography-High Resolution Mass Spectrometry UPLC-HRMS
11:00

Untargeted Metabolomics from Biological Sources Using Ultraperformance Liquid Chromatography-High Resolution Mass Spectrometry UPLC-HRMS

Published on: May 20, 2013

22.4K
Author Spotlight: Optimizing Affinity Chromatography for His-Tagged FEN1 Protein
07:19

Author Spotlight: Optimizing Affinity Chromatography for His-Tagged FEN1 Protein

Published on: April 26, 2024

1.5K

Area of Science:

  • Analytical Chemistry
  • Chromatography
  • Separation Science

Background:

  • High-Performance Liquid Chromatography (HPLC) is a cornerstone of chemical analysis.
  • Capillary Liquid Chromatography (LC) offers advantages like reduced solvent consumption and enhanced sensitivity, particularly with Mass Spectrometry (MS) detection.

Purpose of the Study:

  • To provide practical insights for translating analytical-scale HPLC separations to the capillary scale.
  • To address critical aspects of method development for capillary LC systems.

Main Methods:

  • Examination of pumping systems and detectors suitable for capillary LC.
  • Analysis of extra-column effects impacting separation performance.
  • Detailed review of column selection, including stationary phase options and commercial availability for inner diameters ≤ 0.3 mm.

Main Results:

  • Identification of key factors influencing the successful translation of HPLC methods to capillary scale.
  • Description of strategies for developing capillary LC methods operating at flow rates below 10 μL/min.

Conclusions:

  • Successful implementation of capillary LC requires careful consideration of instrumentation and column parameters.
  • This tutorial provides a foundation for analytical scientists to adopt capillary LC workflows effectively.