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Asymmetric Histone Inheritance Regulates Differential Transcription Re-initiation and Cell Fate Decisions in Mouse
Binbin Ma1,2, Guanghui Yang1,2, Jonathan Yao2
1Howard Hughes Medical Institute, Department of Biology, Johns Hopkins University, Baltimore, MD.
Biorxiv : the Preprint Server for Biology
|March 17, 2025
Summary
Mammalian adult stem cells show asymmetric histone inheritance during division, impacting tissue regeneration and animal behavior. This epigenetic mechanism is crucial for olfactory epithelium repair and function.
Area of Science:
- Epigenetics
- Developmental Biology
- Mammalian Cell Biology
Background:
- Epigenetic inheritance is vital for cellular identity and function in mammals.
- Adult stem cells maintain tissue homeostasis and regeneration through precise cell division.
- Understanding stem cell division and epigenetic regulation is key to regenerative medicine.
Purpose of the Study:
- To investigate asymmetric cell division and histone inheritance in mouse olfactory epithelium stem cells.
- To determine the role of asymmetric histone inheritance in stem cell fate and tissue regeneration.
- To explore the connection between epigenetic inheritance, stemness factors, and animal behavior.
Main Methods:
- Utilized an injury-induced regeneration model of the mouse olfactory epithelium.
- Employed asymmetric cell division and histone inheritance pattern analysis in horizontal basal cells (HBCs).
- Conducted single-cell RNA sequencing on paired daughter cells and analyzed transcription factor enrichment.
Main Results:
- Horizontal basal cells (HBCs) exhibit asymmetric cell division and asymmetric inheritance of histones H4, H3, and H3.3.
- Asymmetric histone inheritance correlates with differential p63 transcription factor enrichment and asynchronous transcription re-initiation.
- Disruption of asymmetric division impairs olfactory epithelium regeneration and alters smell behavior in mice.
Conclusions:
- Asymmetric histone inheritance is a novel epigenetic mechanism in mammalian adult stem cells.
- This mechanism plays a significant role in olfactory epithelium regeneration and sensory behavior.
- Epigenetic inheritance patterns in stem cells are critical for tissue repair and organismal function.
Keywords:
Asymmetric histone inheritanceasymmetric cell divisioncell fate decisionhorizontal basal cellmouse olfactory epitheliumregenerationMore Related Videos
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