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Updated: May 22, 2025

Formulation and Characterization of Bioactive Agent Containing Nanodisks
Published on: March 17, 2023
Gliclazide loaded spanlastic nanovesicles: empowering bioavailability and antidiabetic efficacy
Shimaa Mazyed1, Soha M El-Masry1, Haidy Abbas1
1Department of Pharmaceutics, Faculty of Pharmacy, Damanhour University, Damanhour, Egypt.
Objective:
This work aimed to prepare spanlastics nanovesicles (SNVs) loaded with gliclazide (GCZ) to increase the drug's oral bioavailability and anti-diabetic effects.
Methods:
Two types of edge activators (tween 80 and/or brij35) and two types of spans (span 60 and span 80) were used to prepare SNVs using the ethanol injection method,23 factorial design was used to investigate the effects of various span types, edge activator types, and the ratio of span to edge activator.
Results:
The optimum formulation (F6) was selected and its in-vitro drug release, in-vivo pharmacokinetics, and pharmacodynamics were evaluated. A transition electron microscope (TEM) showed spherical particles with smooth surfaces, (F6) drug release was (Q12 97.05 ± 4.85) while GCZ powder was (97.89 ± 4.56 after 4 h) also showed better entrapment efficiency (EE% 95.1 ± 3.8). In- vivo pharmacokinetic study showed an increase in Cmax and tmax (12.93 ± 1.34, 3.2 ± 0.83) compared to unprocessed GCZ powder (2.88 ± 1.59, 1.8 ± 0.74). In-vivo pharmacodynamics study of diabetic rats demonstrated that GCZ-loaded SNVs has a higher % maximum decrease in blood glucose levels (MR) 58.31 ± 5.70 compared to 38.33 ± 8.18 for free drug and % total drop in blood glucose levels (TD) 25.78 ± 5.31% for GCZ-SNVs compared to 20.26 ± 6.05% for free drug. Histopathological examination revealed no cytotoxic signs in any of the examined samples.
Conclusion:
Results revealed a significant rise in relative bioavailability, sustained and prolonged drug release when compared to the unprocessed GCZ powder.
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