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Updated: May 21, 2025

Two- and Three-Dimensional Live Cell Imaging of DNA Damage Response Proteins
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Pan-cancer genetic profiles of mitotic DNA integrity checkpoint protein kinases

Ayana Meegol Rasteh1,2, Hengrui Liu3,4, Panpan Wang1

  • 1Cancer Research Institute, Jinan University, Guangzhou, China.

Cancer Biomarkers : Section a of Disease Markers
|March 17, 2025
PubMed

Insights

Protein kinases in the DNA integrity checkpoint pathway are implicated in cancer. This study profiles these kinases across 33 cancer types, revealing potential diagnostic and prognostic biomarkers for cancer therapy.

Area of Science:

  • Oncology
  • Genomics
  • Molecular Biology

Background:

  • The mitotic DNA integrity checkpoint signaling pathway is crucial for maintaining genomic stability.
  • Protein kinases play a central role in this pathway, with 16 key genes identified.
  • Dysregulation of this pathway is potentially linked to cancer development.

Purpose of the Study:

  • To generate pan-cancer profiles of 16 protein kinase genes involved in mitotic DNA integrity checkpoint signaling.
  • To investigate their potential as prognostic and diagnostic biomarkers.
  • To identify potential therapeutic targets for cancer treatment.

Main Methods:

  • Acquisition of multi-omic data for 16 protein kinase genes.
  • Analysis of over 9000 samples across 33 cancer types.
  • Profiling of single nucleotide variants (SNV), copy number variations (CNV), methylation, mRNA expression, pathway crosstalk, and microRNA regulation.

Main Results:

  • High SNV mutation frequencies observed in genes like ATM, PRKDC, and PLK1 in specific cancers (e.g., UCEC, SKCM).
  • CNVs of certain genes correlate with patient survival in UCEC, KIRP, and LGG.
  • mRNA expression of these genes may impact BRCA, KIRC, LUAD, and STAD, involving copy number, methylation, and miRNA regulation.

Conclusions:

  • Protein kinases in mitotic DNA integrity checkpoint signaling are implicated in cancer development.
  • These genes show potential as cancer diagnostic and prognostic biomarkers.
  • Further research is needed to validate clinical utility and develop therapeutic applications.
Keywords:
Genetic ProfilesMitotic DNA IntegrityProtein Kinasespan-Cancer

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