Benchtop Proton NMR Spectroscopy for High-Throughput Lipoprotein Quantification in Human Serum and Plasma

We report the successful development and translation of high-field nuclear magnetic resonance (NMR) based comprehensive lipoprotein analysis to routine benchtop systems. This demonstrates the potential to reimagine population level cardiovascular disease risk analysis and individual level screening based on blood sampling. Using a quantitative calibration approach, we obtained stable and reproducible results from multiple sites, despite reduced spectral dispersion and sensitivity at lower field strengths. Our study shows that 25 out of 28 major lipoprotein parameters, including key cardiometabolic risk markers, were faithfully measured using benchtop NMR systems within 15 min. This development has significant implications for making a powerful diagnostic tool widely available, enhancing the potential for longitudinal personalized medicine through molecular phenotyping in the clinic.