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Related Experiment Video

Updated: May 21, 2025

Non-Invasive Visualization of Nailbed Microvascular Morphology in Mice Using Capillaroscopy
05:06

Non-Invasive Visualization of Nailbed Microvascular Morphology in Mice Using Capillaroscopy

Published on: February 28, 2025

160

Non-Invasive Visualization of Nailbed Microvascular Morphology in Mice Using Capillaroscopy.

Olivia L Bossardet1, Clara C Cousins2, Joseph M Holden1

  • 1Department of Ophthalmology and Visual Sciences, Vanderbilt Eye Institute, Vanderbilt University Medical Center.

Journal of Visualized Experiments : Jove
|March 17, 2025
PubMed
Summary

This study presents a simple, non-invasive nailbed capillaroscopy protocol for mice. This technique allows for inexpensive assessment of microvascular morphology in animal models, aiding research translatability.

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Area of Science:

  • Microcirculation research
  • Animal models in disease research
  • Non-invasive imaging techniques

Background:

  • Nailfold capillaroscopy (NFC) is crucial for diagnosing systemic diseases in humans by assessing microvascular function.
  • Translating NFC findings to animal models is difficult due to invasive or expensive methods.
  • There is a need for accessible, non-invasive techniques to study peripheral microvasculature in animal research.

Purpose of the Study:

  • To develop and present a straightforward protocol for nailbed capillaroscopy in mice.
  • To enable easy and cost-effective assessment of microvascular morphology in mouse models.
  • To facilitate the study of peripheral microvascular abnormalities in various mouse disease models.

Main Methods:

  • Application of capillaroscopy technique to the nailbed of mice.
  • Imaging of nailbed microvasculature in wild-type mice (SV129/S6 and C57/B6J strains).
  • Development of a protocol for non-invasive microvascular assessment.

Main Results:

  • Successful application of capillaroscopy to the mouse nailbed.
  • Acquisition of representative images of nailbed microvascular architecture in two common mouse strains.
  • Demonstration of capillaroscopy as a viable non-invasive imaging tool for mouse peripheral microvasculature.

Conclusions:

  • The developed nailbed capillaroscopy protocol offers a simple, inexpensive, and non-invasive method for assessing mouse microvasculature.
  • This technique can significantly aid research in mouse models of diseases involving peripheral microvascular dysfunction.
  • Further studies are warranted to apply this method across diverse mouse disease models.