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Rym Ben-Abdallah1,2, Ines Bouhaoula1,2, Olfa Souissi1
1Parasitology-Mycology Laboratory, Institute Pasteur of Tunis.
La Tunisie Medicale
|March 17, 2025
Summary
The comparative western blot is crucial for diagnosing congenital toxoplasmosis (CT) in newborns. This method, alongside ELISA, aids in confirming CT through antibody detection in infants.
Area of Science:
- Medical Parasitology
- Immunology
- Neonatal Medicine
Context:
- Congenital toxoplasmosis (CT) diagnosis at birth relies heavily on serological methods.
- Neonatal screening for CT is essential to prevent long-term complications.
Purpose:
- To evaluate the effectiveness of biological diagnostic techniques for congenital toxoplasmosis in newborns.
- To report findings from a 17-year study on neonatal CT diagnosis.
Summary:
- A 17-year study analyzed 224 newborns suspected of CT.
- Comparative mother-newborn western blot confirmed CT in 43 newborns (19.2%) via IgG/IgM antibody detection.
- ELISA and IgG persistence also contributed to diagnosis.
Impact:
- Highlights the significant role of comparative western blot in early CT diagnosis.
- Provides data on antibody kinetics (IgG disappearance, IgM positivity) in neonatal CT.
- Informs diagnostic strategies for congenital toxoplasmosis in at-risk infants.