Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Cell Signaling in Plants

Cell Signaling in Plants

Plant cells communicate to coordinate their cycle of growth, flowering and fruiting, and activities in roots, shoots, and leaves in response to the changing environmental conditions. Plant signaling is distinct from animal signaling. Plants primarily utilize enzyme-linked receptors, whereas the largest class of cell-surface receptors in animals are G-protein coupled receptors (GPCRs). Unlike animals, receptor tyrosine kinases are rare in plants. Instead, plants have a diverse class of...

Cell Adhesion in Plants

Cell Adhesion in Plants

Plants have rigid cell walls that are made up of cell wall polysaccharides that mediate cell-cell adhesion. The primary cell walls of plants consist of two independent and interacting polysaccharide networks: a pectin matrix that embeds the second network comprising cellulose and hemicelluloses.
Pectins are complex heteropolymers mainly composed of negatively-charged α-D-glucopyranosyl uronic acid and some neutral glycosyl residues such as α-L-rhamnopyranose, α-L-arabinofuranose,...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

LUX ARRHYTHMO transcription factors regulate photoperiod-mediated resistance against common cutworm in soybean.

Journal of integrative plant biology·2026

Same author

The critical role of the RUNX1/NF-κB transcriptional complex-mediated PVAT-VSMC axis in aortic dissection.

Journal of cardiothoracic surgery·2026

Same author

A GmWRKY20-GmbZIP9 transcriptional module synergistically activates GmANKTM21 to confer drought tolerance in soybean.

Plant physiology·2026

Same author

WMRNN: Weighted Modal Regression Neural Networks for Right Censored Data.

Statistics in medicine·2026

Same author

Pyramiding <i>nn1</i>6 and <i>rin1</i> alleles to balance plant height and node number at high latitudes.

Molecular breeding : new strategies in plant improvement·2026

Same author

Paralogous LRR receptor kinases confer symbiosis specificity between arbuscular mycorrhizal and root nodule symbioses in Lotus japonicus.

The New phytologist·2026

Search research articles

Related Experiment Video

Updated: May 21, 2025

Luciferase Complementation Imaging Assay in Nicotiana benthamiana Leaves for Transiently Determining Protein-protein Interaction Dynamics

Luciferase Complementation Imaging Assay in Nicotiana benthamiana Leaves for Transiently Determining Protein-protein Interaction Dynamics

Published on: November 20, 2017

Evening complex component ELF3 interacts with LUX proteins to repress soybean root nodulation.

Bohong Su¹, Hong Li¹, Ke Zhang¹

¹Guangdong Key Laboratory of Plant Adaptation and Molecular Design, Innovative Center of Molecular Genetic and Evolution, School of Life Science, Guangzhou University, Guangzhou, China.

Plant Biotechnology Journal

|March 17, 2025

Summary

Soybean

Keywords:

ENOD40 INN1–LUX increase nodule number map‐based cloning negative regulation soybean

More Related Videos

Plant Promoter Analysis: Identification and Characterization of Root Nodule Specific Promoter in the Common Bean

Plant Promoter Analysis: Identification and Characterization of Root Nodule Specific Promoter in the Common Bean

Published on: December 23, 2017

Generation of Composite Plants in Medicago truncatula used for Nodulation Assays

Generation of Composite Plants in Medicago truncatula used for Nodulation Assays

Published on: March 27, 2011

Related Experiment Videos

Last Updated: May 21, 2025

Luciferase Complementation Imaging Assay in Nicotiana benthamiana Leaves for Transiently Determining Protein-protein Interaction Dynamics

Luciferase Complementation Imaging Assay in Nicotiana benthamiana Leaves for Transiently Determining Protein-protein Interaction Dynamics

Published on: November 20, 2017

Plant Promoter Analysis: Identification and Characterization of Root Nodule Specific Promoter in the Common Bean

Plant Promoter Analysis: Identification and Characterization of Root Nodule Specific Promoter in the Common Bean

Published on: December 23, 2017

Generation of Composite Plants in Medicago truncatula used for Nodulation Assays

Generation of Composite Plants in Medicago truncatula used for Nodulation Assays

Published on: March 27, 2011

Area of Science:

Plant Biology
Molecular Biology
Genetics

Background:

Root nodule formation is key to legume-rhizobia symbiosis.
Soybean (Glycine max) symbiotic signaling research lags behind model legumes.
Understanding soybean nodulation is crucial for crop improvement.

Purpose of the Study:

To identify genetic factors regulating soybean root nodule formation.
To elucidate the molecular mechanism of soybean nodulation control.
To explore strategies for enhancing biological nitrogen fixation in soybean.

Main Methods:

Map-based cloning to identify the mutated gene.
Gene expression analysis (co-expression).
Genetic and biochemical assays to determine protein complex function.
Promoter binding assays.

Main Results:

A soybean mutant (inn1) with increased root nodules (~25%) was identified.
INN1 encodes the EARLY FLOWERING 3a (ELF3a) protein, part of the Evening Complex.
INN1 forms a complex with LUX1 and LUX2, repressing the pro-nodulation gene ENOD40, thus negatively regulating nodulation.
Knockout/knockdown of INN1/LUX1/LUX2 enhances nodulation.

Conclusions:

Soybean ELF3a/INN1 negatively regulates root nodule formation.
The INN1-LUX complex controls nodulation by repressing ENOD40 expression.
This finding provides a basis for improving soybean's biological nitrogen fixation capacity.