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Updated: May 21, 2025
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FISH for Pre-implantation Genetic Diagnosis
Published on: February 23, 2011
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'When Lightning Strikes Twice'-Preimplantation Genetic Testing for Two Indications in One Biopsy
Einav Kremer1, Hagit Daum2, Amy Solnica3
1IVF Unit, Department of Obstetrics and Gynecology, Hadassah Medical Organization and Faculty of Medicine, The Hebrew University, Jerusalem, Israel.
Prenatal Diagnosis
|March 17, 2025
Summary
Preimplantation genetic testing for two indications (PGT2) is feasible and shows similar live birth rates compared to testing for one indication (PGT1). PGT2 results in fewer suitable embryos for transfer per cycle.
Area of Science:
- Reproductive Medicine
- Genetics
- Infertility Treatment
Background:
- Preimplantation genetic testing (PGT) is crucial for identifying genetic abnormalities in embryos before implantation.
- Evaluating the efficiency of PGT for multiple genetic indications versus a single indication is essential for optimizing treatment protocols.
Purpose of the Study:
- To compare clinical pregnancy and live birth rates between PGT for two indications (PGT2) and PGT for one autosomal dominant indication (PGT1).
Main Methods:
- A retrospective cohort study analyzed data from 44 PGT patients (2015-2023).
- Patients were divided into PGT2 (22 patients, 113 cycles) and PGT1 (22 patients, 108 cycles) groups.
- Statistical analyses included t-tests, Mann-Whitney U tests, mixed models, and logistic regressions.
Main Results:
- No significant difference in clinical pregnancy or live birth rates between PGT1 and PGT2 groups.
- PGT2 patients had more fresh embryos but fewer frozen embryos post-biopsy compared to PGT1.
- PGT2 group had fewer embryos available for transfer per cycle (1.30 vs. 1.89).
Conclusions:
- Testing for two genetic indications in a single biopsy is clinically feasible.
- PGT2 yields a lower proportion of embryos suitable for transfer but maintains similar live birth rates to PGT1.
