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Updated: May 21, 2025

Synthesis of an Intein-mediated Artificial Protein Hydrogel
Published on: January 27, 2014
A novel simplified structural design as an artificial enzyme for efficient hydrolysis of PNPA
Wenfang Li1, Yuze Lu2, Jiajun Wang2
1Key Laboratory of Preparation and Application of Environmentally Friendly Materials, Ministry of Education, College of Chemistry, Jilin Normal University, Changchun, 130103, China. liwf12@jlnu.edu.cn.
Abstract:
In this study, a novel artificial enzyme Zn(II)-SMM with simple organic structure for PNPA-directed hydrolysis is proposed. The introduction of hydrophobic quinoline and dipyridinium in Zn(II)-SMM system demonstrates high activity for the complexation of Zn2+ and hydrolysis of PNPA, and the catalytic rate (kcat/kuncat) exceeds 5239 times that of non-catalytic systems. The newly designed small molecule organic complex effectively binds to and catalyzes the hydrolysis of substrate PNPA in accordance with Michaelis-Menten kinetics. The mechanism with activation by single Lewis acid provides fundamental insights for the development of small molecule hydrolases.
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