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Aerobic oxidation catalyst vanadyl acetylacetonate boosts luminol chemiluminescence for sensitive detection of
Wei Zhang1,2, Yequan Chen2, Kaixiang Ji2
1School of Applied Chemistry and Engineering, University of Science and Technology of China, Hefei, 230026, Anhui, China.
Abstract:
Vanadyl acetylacetonate, VO(acac)2, is a well-known environmentally friendly catalyst for aerobic oxidations. It has been exploited as an efficient co-reactant of luminol chemiluminescence (CL) in aqueous media for the first time. The luminol-VO(acac)2 system generates an intense CL and ascorbic acid can quench its CL remarkably. Based on the strong quenching effect of ascorbic acid, the luminol-VO(acac)2 CL was used in the quantification of clinically important ascorbic acid and alkaline phosphatase (ALP) by enzymatic conversion of L-ascorbic acid 2-phosphate into ascorbic acid. The linear ranges for detecting ascorbic acid and alkaline phosphatase are 1 to 100 μM and 0.005 to 200 U/L, respectively. The limits of detection of ascorbic acid and alkaline phosphatase are 0.5 μM and 0.005 U/L, respectively. The proposed CL method has shown high selectivity for the ALP among the tested biomolecules and metal ions. It also shows good recoveries in human serum samples.
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