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Sustained release of Sr and Ca from a micronanotopographic titanium surface improves osteoblast function
Luan Viana Faria1, Maria Eduarda Scordamaia Lopes1, Diego Pedreira de Oliveira2
1São Paulo State University (UNESP), School of Dentistry at Araraquara, Araraquara, São Paulo, Brazil.
Summary
Strontium (Sr) and calcium (Ca) doping on micronanotopographic titanium implants enhances bone cell activity and mineralization. Sr-doped surfaces alone significantly improve osseointegration compared to untreated or undoped implants.
Area of Science:
- Biomaterials Science
- Dental Implantology
- Tissue Engineering
Background:
- Surface properties of dental implants critically influence osseointegration.
- Alkaline earth elements like strontium (Sr) and calcium (Ca) promote bone formation.
Purpose of the Study:
- To evaluate micronanotopographic cpTi substrates doped with Sr and Ca.
- To assess the impact of these doped surfaces on pre-osteoblast behavior and osseointegration.
Main Methods:
- Characterization using XPS, SEM, AFM; wettability, release, and electrochemical tests.
- In vitro evaluation of MC3T3-E1 cell proliferation, adhesion, differentiation, viability, ALP activity, and mineralization.
- Analysis of osteoblastic gene (Bglap, Ibsp, Spp, Col1a1, Runx2) and protein expression via PCR and ELISA.
Main Results:
- Doped surfaces showed no cytotoxicity, increased cell spreading, and enhanced mineralization.
- Sr and Sr/Ca doped surfaces significantly improved mineralization compared to controls.
- Osteoblastic gene and protein expression were regulated on doped surfaces.
Conclusions:
- Micronanotopographic substrates doped with Sr and Sr/Ca enhance physicochemical properties and pre-osteoblast response.
- Sr and Sr/Ca doped implants exhibit superior biological effects for osseointegration.
- Sr doping alone is sufficient to improve osseointegration-related events.
