Enhanced microbial degradation of hexabromocyclododecane in riparian sediments through regulating flooding regimes

Hexabromocyclododecane (HBCD), a persistent halogenated organic pollutant, has been commonly detected in river sediments, especially in riparian zones, but strategies for promoting its microbial degradation remain insufficiently explored. This study hypothesized that regulating the flooding regime of sediments could accelerate microbial degradation of HBCD in riparian zones and evaluated the underlying mechanisms. Results showed that, compared with high-frequency flooding-drying or no alternations, the low-frequency flooding-drying alternation (6 weeks of flooding and 6 weeks of drying, 6F:6D) significantly promoted microbial degradation of HBCD. This may be due to changes in sediment redox potential under the 6F:6D regime, facilitating the sequential reductive debromination and aerobic degradation process of HBCD. The abundances of organohalide-respiring bacteria (Dehalococcoides spp. and Dehalogenimonas spp.) were always high in the 6F:6D regime, irrespective of flooding or drying periods. Furthermore, the complex bacterial co-occurrence patterns, specific ecological clusters, and potential keystone species including the genera Methylibium, Nitrospira, and Dehalococcoides, may play important degradative roles of HBCD in the 6F:6D regime. Overall, microbial degradation of HBCD can be promoted under low-frequency flooding-drying alternation regulated by hydraulic structures, providing an effective and eco-friendly strategy for ecological restoration.