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Cryo-electron Microscopy01:28

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Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...
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Related Experiment Video

Updated: May 21, 2025

Author Spotlight: Preservation of Bioenergetic Parameters in Peripheral Blood Mononuclear Cells After Cryopreservation
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Evaluating Cryopreservation Methods in Biobanking: Impacts on Biomarker Integrity and Omics Data Reliability.

Annamaria Antona1, Valentina Bettio1,2, Jacopo Venetucci1

  • 1Department of Translational Medicine (DIMET), Center of Excellence in Aging Sciences, University of Piemonte Orientale, Novara, Italy.

Biopreservation and Biobanking
|March 18, 2025
PubMed
Summary

Biobanking storage methods, cryovials and straws, are suitable for standard analyses. However, omics data may show subtle variations depending on the cryopreservation container used for plasma and serum samples.

Keywords:
biobankingblood biomarkerscryopreservationstrawsvials

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Area of Science:

  • Biomedical Research
  • Biobanking
  • Personalized Medicine

Background:

  • Personalized medicine relies on biobanks for high-quality biospecimens.
  • Optimized protocols are crucial for sample integrity in biobanking.
  • Evaluating long-term storage conditions is essential for reliable research data.

Purpose of the Study:

  • To assess the impact of cryopreservation methods (cryovials vs. straws) on plasma and serum sample integrity.
  • To compare the suitability of different storage methods for various analytical techniques.
  • To provide insights for optimizing biobanking practices.

Main Methods:

  • Plasma and serum samples were cryopreserved in liquid nitrogen using cryovials and straws.
  • Samples underwent standard biochemical analysis, targeted lipidomics, untargeted proteomics, and targeted metabolomics.
  • Mass spectrometry-based techniques were employed for omics analyses.

Main Results:

  • Both cryovials and straws proved robust for standard laboratory analyses of plasma and serum.
  • Lipidomic analysis showed minor lipid abundance disparities, mainly in fatty acids.
  • Proteomic and metabolomic analyses revealed significant variations in a subset of analytes between storage methods.

Conclusions:

  • Cryovials and straws are largely equivalent for robust, low-sensitivity analyses.
  • Caution is advised when interpreting omics data from samples stored using different cryopreservation methods.
  • Understanding storage method impact is key for reliable biospecimen data in research.