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Updated: May 21, 2025
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Investigating Intestinal Inflammation in DSS-induced Model of IBD
Published on: February 1, 2012
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Bacterial dysbiosis and decrease in SCFA correlate with intestinal inflammation following alcohol intoxication and
Caroline J Herrnreiter1,2,3,4, Mary Grace Murray1,2,3,4, Marisa Luck1,2,4,5
1Burn and Shock Trauma Research Institute, Loyola University Chicago Health Sciences Division, Maywood, Illinois, USA.
Egastroenterology
|March 18, 2025
Summary
Alcohol and burn injury disrupt the gut microbiome, decreasing beneficial short-chain fatty acids (SCFAs). This dysbiosis promotes intestinal inflammation and barrier dysfunction, increasing post-burn complications.
Area of Science:
- Microbiology
- Gastroenterology
- Trauma Surgery
Background:
- Alcohol intoxication at the time of burn injury is associated with increased sepsis and mortality.
- Understanding the gut microbiome's role in alcohol-related burn injury is crucial for improving patient outcomes.
Purpose of the Study:
- To investigate the impact of alcohol intoxication and burn injury on the intestinal microbiome and short-chain fatty acids (SCFAs).
- To determine the association between these changes and intestinal inflammation.
Main Methods:
- Mice underwent ethanol intoxication and scald burn injury.
- Faecal samples were analyzed for microbial composition (16S sequencing) and SCFA levels (HPLC-MS).
- In vitro assays assessed the inflammatory response of intestinal cells to faecal slurries.
Main Results:
- Ethanol burn (EB) mice showed decreased microbial diversity and increased Proteobacteria compared to controls.
- EB mice had significantly lower butyrate and a trend towards lower acetate and total SCFAs.
- Faecal slurries from EB mice induced higher interleukin-6 (IL-6) release from intestinal cells, which was reduced by butyrate supplementation.
Conclusions:
- Alcohol and burn injury induce gut dysbiosis and reduce SCFAs.
- These changes contribute to intestinal inflammation and barrier disruption, potentially worsening post-injury outcomes.
Keywords:
Alcohol MetabolismBacterial DysbiosisGastrointestinal MicrobiomeInflammationInterleukin-6Short-chain Fatty AcidsMore Related Videos
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