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Transplantation and Monitoring of a Murine Minor Histocompatibility Antigen-Mismatched Model of Graft-Versus-Host

Amir Shamshirian1,2, Karshing Chang1, Kate H Gartlan3,4

  • 1Immunopathology Laboratory, Infection & Inflammation Program, QIMR Berghofer Medical Research Institute, Herston, Brisbane, QLD, Australia.

Methods in Molecular Biology (Clifton, N.J.)
|March 18, 2025
PubMed

Murine models of allogeneic stem cell transplantation (allo-SCT) have been a valuable tool in expanding our understanding of graft-versus-host disease (GVHD) immunopathology, providing a platform to manipulate allo-SCT and mimic the clinical scenarios observed in humans. These systems have been instrumental in advancing our understanding of the disease mechanisms and facilitating the exploration of novel therapeutic strategies. This chapter outlines the transplantation and monitoring methods in a murine minor histocompatibility antigen-mismatched model of GVHD model using C3H.SW donors and C57BL/6J recipients, aiming to provide researchers with a comprehensive guide to effectively use this model for advancing GVHD treatment and improving patient outcomes in allo-SCT.

Keywords:
AllotransplantationBone marrow transplantationFlow cytometryGraft-versus-host diseaseMagnetic bead separationMouseStem cell transplantationT cell

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