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Related Concept Videos

In Vitro Fertilization01:24

In Vitro Fertilization

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In vitro fertilization (IVF) is a form of assisted reproductive technology where an egg is fertilized with sperm in a controlled laboratory environment before transferring the resulting embryo into the uterus. This process is designed to help individuals and couples experiencing difficulties conceiving.
The IVF process begins with ovarian stimulation, during which reproductive endocrinologists prescribe hormonal medications to stimulate the ovaries to produce multiple eggs instead of the single...
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Infertility in Males01:23

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Male infertility affects millions of couples worldwide, arising from various factors that impact different stages of the reproductive process. An endocrine imbalance resulting from conditions like hypogonadism, Klinefelter syndrome, or pituitary disorders can disrupt hormone levels and reduce sperm production. Testicular defects, such as tumors, cryptorchidism, atrophic testes, abnormal sperm morphology, and low sperm count or motility, may arise due to genetic factors, structural...
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Related Experiment Video

Updated: May 21, 2025

Fertility Preservation Through Oocyte Vitrification: Clinical and Laboratory Perspectives
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Andrology laboratory techniques for micro-TESE/IVF/ICSI: a narrative review.

Krishna Chaitanya Mantravadi1, Christina Anagnostopoulou2, Firuza R Parikh3

  • 1Department of Clinical Embryology, Oasis Fertility, Hyderabad 500034, Telangana, India.

Asian Journal of Andrology
|March 18, 2025
PubMed
Summary

Sperm processing is crucial for assisted reproductive technology (ART) success, removing detrimental factors like dead sperm and leukocytes. Optimized techniques improve outcomes for procedures like in vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI).

Keywords:
andrology laboratory techniquesintracytoplasmic sperm injectionmicro-testicular sperm extraction

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Area of Science:

  • Reproductive Biology
  • Andrology
  • Assisted Reproductive Technology

Background:

  • Sperm processing is vital in assisted reproductive technology (ART) to isolate viable sperm.
  • Seminal plasma removal and capacitation are key aims.
  • Leukocytes, bacteria, and dead spermatozoa can negatively impact oocyte fertilization and embryo development due to oxidative stress.

Purpose of the Study:

  • To highlight the importance of sperm processing in ART.
  • To discuss the removal of detrimental factors from semen.
  • To outline the varying requirements of different ART procedures.

Main Methods:

  • Review of current sperm processing techniques in ART.
  • Discussion of sperm selection criteria for IVF, ICSI, and TESE.
  • Emphasis on sperm cryopreservation for TESE cases.

Main Results:

  • Effective sperm processing removes leukocytes, bacteria, and dead spermatozoa, mitigating oxidative stress.
  • Optimized techniques yield more motile, morphologically normal sperm essential for IVF success.
  • Specialized sperm selection is critical for ICSI, and careful processing is needed for TESE.

Conclusions:

  • Sperm processing is indispensable for successful ART outcomes.
  • Tailored techniques are necessary for different procedures like IVF, ICSI, and TESE.
  • Proper sperm processing and cryopreservation enhance the efficiency and success rates of ART.