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Related Experiment Video

Updated: May 21, 2025

Using Nanoplasmon-Enhanced Scattering and Low-Magnification Microscope Imaging to Quantify Tumor-Derived Exosomes
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Dual-color fluorescence detection of tumor-derived extracellular vesicles using a specific and serum-stable

Huimin Kong1, Xiaodie Chen1, Weijen Lee1

  • 1Laboratory of Biomaterials and Translational Medicine, Center for Nanomedicine, The Third Affiliated Hospital, Sun Yat-sen University, Guangzhou 510630, China.

Biosensors & Bioelectronics
|March 18, 2025
PubMed
Summary
This summary is machine-generated.

This study introduces a novel dual-biomarker assay for detecting tumor-derived extracellular vesicles (tEVs) in liquid biopsies. The method simultaneously quantifies EpCAM protein and miR-21 levels, significantly improving cancer diagnostic accuracy.

Keywords:
CRISPR/Cas13aCancer diagnosisMembrane fusionMicroRNATumor-derived extracellular vesicle

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Area of Science:

  • Biochemistry
  • Molecular Biology
  • Nanotechnology

Background:

  • Tumor-derived extracellular vesicles (tEVs) are key in cancer progression and hold diagnostic potential for liquid biopsies.
  • Current methods for tEV biomarker detection (ELISA, qRT-PCR) lack sensitivity, specificity, and are complex.
  • Existing biosensors detect only single biomarkers, risking misdiagnosis.

Purpose of the Study:

  • To develop a novel assay for simultaneous detection of tEV protein and microRNA biomarkers.
  • To enhance the accuracy and efficiency of cancer liquid biopsy through a dual-biomarker approach.

Main Methods:

  • Developed a specific and serum-stable membrane-fusion approach (SSMFA) using aptamer-modified liposomes.
  • SSMFA simultaneously quantifies EpCAM protein via FRET-based red fluorescence and miR-21 via CRISPR/Cas13a-mediated green fluorescence.
  • Utilized dual-color fluorescence analysis for simultaneous biomarker detection.

Main Results:

  • SSMFA accurately quantified EpCAM protein and miR-21 levels in tEVs from hepatocellular carcinoma models.
  • The assay requires minimal sample volume and has a rapid assay time of 2 hours.
  • Dual-biomarker detection showed a strong correlation with tumor burden and improved diagnostic accuracy (AUC = 0.98).

Conclusions:

  • SSMFA offers a sensitive, specific, and efficient method for simultaneous tEV biomarker detection.
  • This dual-biomarker approach significantly enhances cancer diagnostic accuracy in liquid biopsies.
  • SSMFA represents a promising advancement for tEV-based liquid biopsy assays.