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Strigolactone insensitivity affects the hormonal homeostasis in barley
Magdalena Korek1, Devang Mehta2, Glen R Uhrig2
1Institute of Biology, Biotechnology and Environmental Protection, Faculty of Natural Sciences, University of Silesia in Katowice, Jagiellonska 28, 40-032, Katowice, Poland. magdalena.korek@us.edu.pl.
Scientific Reports
|March 19, 2025
Summary
Plant architecture is regulated by strigolactones (SL). A barley HvD14 gene mutation, affecting SL signaling, resulted in increased tillering and altered hormone levels, revealing new insights into plant development.
Area of Science:
- Plant Biology
- Molecular Biology
- Agronomy
Background:
- Plant architecture, crucial for yield, is regulated by hormones like strigolactones (SL).
- SLs inhibit axillary outgrowth, controlling plant branching.
- Mutations in SL receptors, like HvD14 in barley, significantly impact plant architecture.
Purpose of the Study:
- To elucidate the regulatory mechanism behind increased tillering in SL-insensitive barley plants (hvd14 genotype).
- To investigate hormone profiling, transcriptomic, and proteomic changes in hvd14 mutants compared to wild-type (WT).
Main Methods:
- Hormone profiling of WT and hvd14 barley plants.
- Transcriptomic and proteomic analyses of 2- and 4-week-old plants.
- Bioinformatics analysis to identify differentially expressed genes (DEGs) and abundant proteins (DAPs).
Main Results:
- The hvd14 mutant exhibited significantly increased cytokinin and decreased auxin and abscisic acid levels.
- 11.6% of DEGs and 14.6% of DAPs were associated with phytohormone-related processes.
- Potential SL-dependent transcription factors (TFs) were identified, some conserved across monocots and dicots.
Conclusions:
- Altered hormone levels and identified TFs likely mediate the increased tillering in SL-insensitive barley.
- The findings provide insights into SL signal transduction conserved between barley and Arabidopsis thaliana.
- This research contributes to understanding plant architectural regulation for agronomic improvement.
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