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Related Experiment Videos

An indwelling cannula system for the primate eye.

K Miki, R Patterson, S J Ryan

    Journal of Neuroscience Methods
    |May 1, 1985
    PubMed
    Summary

    A novel indwelling cannula system enables safe, chronic drug delivery to the monkey eye's vitreous cavity. This system allows for repeated intravitreal injections or continuous infusion without adverse effects, facilitating retinal drug distribution.

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    Glycine at the 65th position plays an essential role in ATP-dependent protein folding by Archael group II chaperonin.

    Biochemical and biophysical research communications·2001

    Area of Science:

    • Ophthalmology
    • Biomedical Engineering
    • Pharmacology

    Background:

    • Chronic ocular diseases require sustained drug delivery to the vitreous cavity.
    • Current methods for intravitreal drug administration have limitations in achieving long-term therapeutic levels.
    • Developing a reliable system for chronic intravitreal drug infusion is crucial for effective ocular treatment.

    Purpose of the Study:

    • To design and evaluate an indwelling cannula system for chronic drug infusion into the vitreous cavity.
    • To assess the safety and efficacy of the system in a non-human primate model.
    • To investigate drug distribution patterns following repeated injections and continuous infusion.

    Main Methods:

    • An indwelling cannula system was surgically implanted into the pars plana of cynomolgus monkey eyes.
    • The system was tested with repeated injections and continuous infusion via a pumping device.
    • Clinical and histopathological examinations were performed up to 18 months post-implantation.
    • Autoradiography using tritiated leucine was employed to track drug distribution.

    Main Results:

    • No adverse clinical effects were observed up to 18 months after implantation.
    • Histopathology showed minimal, localized tissue proliferation at the wound site, with normal retinal and choroidal morphology.
    • Autoradiography confirmed successful drug delivery, with distinct labeling patterns for injections versus continuous infusion.
    • Evidence suggests drugs infused into the vitreous readily spread into the retina.

    Conclusions:

    • The indwelling cannula system is safe and feasible for repeated intravitreal injections and chronic drug delivery.
    • The system effectively delivers therapeutic agents to the vitreous cavity without causing significant ocular damage.
    • Drug distribution studies indicate efficient retinal penetration, supporting its potential for treating posterior segment eye diseases.

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