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Intact DNA strands can be found in fossils, while scientists sometimes struggle to keep RNA intact under laboratory conditions. The structural variations between RNA and DNA underlie the differences in their stability and longevity. Because DNA is double-stranded, it is inherently more stable. The single-stranded structure of RNA is less stable but also more flexible and can form weak internal bonds. Additionally, most RNAs in the cell are relatively short, while DNA can be up to 250 million...
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The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the...
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Updated: May 21, 2025

A Method for Measuring RNA N6-methyladenosine Modifications in Cells and Tissues
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m6A2Circ: A comprehensive database for decoding the regulatory relationship between m6A modification and circular

Yongtian Li1,2, Bianli Gu3, Lixia Ma3

  • 1Department of Hematology, The First Affiliated Hospital of Chongqing Medical University, School of Basic Medical Sciences, Chongqing Medical University, Chongqing 400016, China.

Computational and Structural Biotechnology Journal
|March 19, 2025
PubMed
Summary

This study introduces m6A2Circ, a database detailing N6-methyladenosine (m6A) modifications in circular RNAs (circRNAs). It provides extensive data on m6A-circRNA interactions to advance research in this field.

Keywords:
Circular RNADatabaseN6-methyladenosine

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Identification of Circular RNAs using RNA Sequencing
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Area of Science:

  • Molecular Biology
  • Genomics
  • Bioinformatics

Background:

  • Circular RNAs (circRNAs) are noncoding RNAs formed by pre-mRNA back-splicing.
  • N6-methyladenosine (m6A) modification is increasingly recognized for its regulatory roles in circRNA metabolism.
  • A comprehensive resource for m6A-circRNA interactions is lacking.

Purpose of the Study:

  • To develop a dedicated database, m6A2Circ, for exploring m6A modification in circRNAs.
  • To systematically catalog m6A-circRNA regulatory interactions.
  • To facilitate research on circRNA function and disease relevance.

Main Methods:

  • Compilation of m6A-circRNA associations from human and mouse tissues.
  • Categorization of associations based on experimental and high-throughput sequencing data.
  • Development of a web-accessible database (http://m6a2circ.canceromics.org/).

Main Results:

  • The m6A2Circ database contains 198,804 m6A-circRNA associations.
  • Associations are supported by four levels of evidence.
  • The database provides extensive annotations for circRNA research.

Conclusions:

  • m6A2Circ addresses the need for a comprehensive resource on m6A modification in circRNAs.
  • The database supports research into the functional and disease implications of m6A-circRNA crosstalk.
  • m6A2Circ aims to foster novel discoveries in RNA epigenetics and noncoding RNA biology.