Related Experiment Video
Updated: May 21, 2025

A Simple Fractionated Extraction Method for the Comprehensive Analysis of Metabolites, Lipids, and Proteins from a Single Sample
Published on: June 1, 2017
Fraction collection of bioactive compounds from ion chromatography: No longer mission impossible
Hans S A Yates1,2, James F Carter1, Mary T Fletcher2
1Queensland Health Coronial and Public Health Sciences (QHCPHS) Health and Food Sciences Precinct, Coopers Plains, QLD, Australia.
Abstract:
This paper demonstrates the fraction collection of the novel sugar trehalulose, using a modified ion chromatograph. The Ion Chromatography (IC) method, previously published for the analysis of trehalulose, was augmented with a suppressor and purpose-made switching valve unit. A sample of stingless bee honey was then run, following the three main steps:•Separation of trehalulose fraction•Lyophilization•Confirmation of trehalulose The method should be applicable to not only sugar analysis but to any bioactive compound separable by IC. The authors were not able to find a similar method within currently published literature.
More Related Videos
09:57Separation of Bioactive Small Molecules, Peptides from Natural Sources and Proteins from Microbes by Preparative Isoelectric Focusing IEF Method
Published on: June 14, 2020
11:25Multi-step Preparation Technique to Recover Multiple Metabolite Compound Classes for In-depth and Informative Metabolomic Analysis
Published on: July 11, 2014
Related Concept Videos
Affinity Chromatography
Principles Of Column Chromatography
Types Of Column Chromatography
Gel Filtration Chromatography
When the...
Analyte Adsorption and Distribution
High-Performance Liquid Chromatography: Introduction
In HPLC, two phases play a critical role in the separation process:
Subcellular Fractionation
Differential Centrifugation
Differential centrifugation is...