Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same authorSame journal

miR-486-5p and miR-144-3p as candidate regulators of cortisol biosynthesis: functional and transcriptomic evidence in adrenocortical cells.

European journal of endocrinology·2026

Same author

International Delphi-based Consensus: Therapeutic Approaches for Pediatric Adrenocortical Carcinoma.

European journal of endocrinology·2026

Same author

The renin-angiotensin system is activated by short-term, but repressed by long-term growth hormone excess.

European journal of endocrinology·2026

Same author

Three-week actigraphy to assess sleep behaviour and circadian rest-activity patterns in suspected and confirmed Cushing's syndrome: an exploratory prospective cohort study.

Pituitary·2026

Same author

GLP-1 Receptor Agonist Exposure and Malignancy Risk in Patients With Endogenous Cushing's Syndrome.

The Journal of clinical endocrinology and metabolism·2026

Same author

Aldosterone-related biochemical phenotypes in adrenal incidentalomas: clinical relevance in a cohort including normotensive patients.

Journal of endocrinological investigation·2026

Search research articles

Related Experiment Video

Updated: May 21, 2025

MicroRNA Based Liquid Biopsy: The Experience of the Plasma miRNA Signature Classifier MSC for Lung Cancer Screening

MicroRNA Based Liquid Biopsy: The Experience of the Plasma miRNA Signature Classifier MSC for Lung Cancer Screening

Published on: October 26, 2017

Identification of hypertension subtypes using microRNA profiles and machine learning.

Smarti Reel¹, Parminder S Reel¹, Josie Van Kralingen²

¹Division of Population Health and Genomics, School of Medicine, University of Dundee, Dundee DD2 4BF, United Kingdom.

European Journal of Endocrinology

|March 19, 2025

Summary

This summary is machine-generated.

This study shows that circulating microRNAs (miRNAs) can help distinguish endocrine hypertension (EHT) from primary hypertension (PHT). Machine learning identified specific miRNAs for accurate EHT subtype diagnosis.

Keywords:

diagnostics hypertension machine learning microRNA

More Related Videos

Tissue-specific miRNA Expression Profiling in Mouse Heart Sections Using In Situ Hybridization

Tissue-specific miRNA Expression Profiling in Mouse Heart Sections Using In Situ Hybridization

Published on: September 15, 2018

MicroRNA In situ Hybridization for Formalin Fixed Kidney Tissues

MicroRNA In situ Hybridization for Formalin Fixed Kidney Tissues

Published on: November 30, 2013

Related Experiment Videos

Last Updated: May 21, 2025

MicroRNA Based Liquid Biopsy: The Experience of the Plasma miRNA Signature Classifier MSC for Lung Cancer Screening

MicroRNA Based Liquid Biopsy: The Experience of the Plasma miRNA Signature Classifier MSC for Lung Cancer Screening

Published on: October 26, 2017

Tissue-specific miRNA Expression Profiling in Mouse Heart Sections Using In Situ Hybridization

Tissue-specific miRNA Expression Profiling in Mouse Heart Sections Using In Situ Hybridization

Published on: September 15, 2018

MicroRNA In situ Hybridization for Formalin Fixed Kidney Tissues

MicroRNA In situ Hybridization for Formalin Fixed Kidney Tissues

Published on: November 30, 2013

Area of Science:

Biomarker discovery
Genomics
Cardiovascular research

Background:

Hypertension affects 1 in 3 adults, with primary hypertension (PHT) being most common.
Endocrine hypertension (EHT), ~10% of cases, arises from conditions like primary aldosteronism (PA), Cushing's syndrome (CS), or pheochromocytoma/paraganglioma (PPGL).
EHT is often misdiagnosed, delaying treatment and leading to ineffective management.

Purpose of the Study:

To identify circulating microRNA (miRNA) biomarkers for distinguishing EHT and its subtypes from PHT.
To evaluate the effectiveness of machine learning (ML) in classifying hypertension types using miRNA profiles.

Main Methods:

Systematic analysis of circulating miRNA features.
Application of 8 supervised machine learning methods for classification and prediction.
Utilizing miRNA data to differentiate EHT subtypes (PA, CS, PPGL) from PHT.

Main Results:

Machine learning models achieved high accuracy: AUC of 0.9 for classifying PPGL, CS, and EHT from PHT.
Models achieved AUC of 0.8 for distinguishing PA from PHT.
Key circulating miRNAs identified include hsa-miR-15a-5p and hsa-miR-32-5p.

Conclusions:

Circulating miRNAs show significant potential as diagnostic biomarkers for EHT.
Machine learning is a viable tool for identifying informative miRNAs for hypertension diagnosis.